| Literature DB >> 16214501 |
Dominic E Warrino1, Louis J DeGennaro, Mary Hanson, Susan Swindells, Samuel J Pirruccello, Wayne L Ryan.
Abstract
We evaluated whole blood samples drawn from 25 healthy donors and 20 HIV-infected donors into K3EDTA and Cyto-Chex BCT blood collection tubes for CD4, CD8, and CD3 cell counts (HIV Panel). Samples collected in Cyto-Chex BCT were stored at room temperature and tested by 4-color flow cytometry at 6 h, 3 days, and 7 days after isolation for CD4, CD8, and CD3 absolute cell counts/microl and compared with samples collected in K3EDTA tubes and tested at 6 h. Regardless of donor type, the samples collected in Cyto-Chex BCT and tested on day 7 yielded results that were statistically indistinguishable (with correlation coefficients of 0.96 or greater) from samples collected in K3EDTA tubes and tested at 6 h. We conclude that whole blood samples collected in Cyto-Chex BCT are stabilized for their marker phenotype for at least 7 days after phlebotomy.Entities:
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Year: 2005 PMID： 16214501 DOI： 10.1016/j.jim.2005.01.021
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303