| Literature DB >> 16212435 |
Christian Melle1, Günther Ernst, Bettina Schimmel, Annett Bleul, Roland Kaufmann, Merten Hommann, Konrad K Richter, Wolfgang Daffner, Utz Settmacher, Uwe Claussen, Ferdinand von Eggeling.
Abstract
We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 x 10(-6)) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry.Entities:
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Year: 2005 PMID： 16212435 DOI： 10.1021/pr050123o
Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466