Literature DB >> 16211513

Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overview.

Reinhard Grisshammer1, Jim F White, Loc B Trinh, Joseph Shiloach.   

Abstract

Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands.

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Year:  2005        PMID： 16211513     DOI： 10.1007/s10969-005-1917-6

Source DB:  PubMed          Journal:  J Struct Funct Genomics        ISSN： 1345-711X


  44 in total

1.  Functional coupling with Galpha(q) and Galpha(i1) protein subunits promotes high-affinity agonist binding to the neurotensin receptor NTS-1 expressed in Escherichia coli.

Authors:  R Grisshammer; E Hermans
Journal:  FEBS Lett       Date:  2001-03-30       Impact factor: 4.124

2.  Conformation of ligands bound to the muscarinic acetylcholine receptor.

Authors:  Hiroyasu Furukawa; Toshiyuki Hamada; Mariko K Hayashi; Tatsuya Haga; Yutaka Muto; Hiroshi Hirota; Shigeyuki Yokoyama; Kazuo Nagasawa; Masaji Ishiguro
Journal:  Mol Pharmacol       Date:  2002-10       Impact factor: 4.436

3.  Automated large-scale purification of a G protein-coupled receptor for neurotensin.

Authors:  Jim F White; Loc B Trinh; Joseph Shiloach; Reinhard Grisshammer
Journal:  FEBS Lett       Date:  2004-04-30       Impact factor: 4.124

4.  Purification of a rat neurotensin receptor expressed in Escherichia coli.

Authors:  J Tucker; R Grisshammer
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

5.  Engineering of a proteolytically stable human beta 2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells.

Authors:  W Hampe; R H Voss; W Haase; F Boege; H Michel; H Reiländer
Journal:  J Biotechnol       Date:  2000-02-17       Impact factor: 3.307

Review 6.  Neurotensin and neurotensin receptors.

Authors:  J P Vincent; J Mazella; P Kitabgi
Journal:  Trends Pharmacol Sci       Date:  1999-07       Impact factor: 14.819

7.  Expression of functional M2 muscarinic acetylcholine receptor in Escherichia coli.

Authors:  H Furukawa; T Haga
Journal:  J Biochem       Date:  2000-01       Impact factor: 3.387

8.  Expression of delta, kappa and mu human opioid receptors in Escherichia coli and reconstitution of the high-affinity state for agonist with heterotrimeric G proteins.

Authors:  L Stanasila; D Massotte; B L Kieffer; F Pattus
Journal:  Eur J Biochem       Date:  1999-03

9.  Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli.

Authors:  H Markus Weiss; Reinhard Grisshammer
Journal:  Eur J Biochem       Date:  2002-01

10.  Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1.

Authors:  Jean-Louis Baneres; Aimée Martin; Pierre Hullot; Jean-Pierre Girard; Jean-Claude Rossi; Joseph Parello
Journal:  J Mol Biol       Date:  2003-06-13       Impact factor: 5.469
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  20 in total

1.  Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein.

Authors:  A Korepanova; J D Moore; H B Nguyen; Y Hua; T A Cross; F Gao
Journal:  Protein Expr Purif       Date:  2006-12-24       Impact factor: 1.650

2.  Structure of a double transmembrane fragment of a G-protein-coupled receptor in micelles.

Authors:  Alexey Neumoin; Leah S Cohen; Boris Arshava; Subramanyam Tantry; Jeffrey M Becker; Oliver Zerbe; Fred Naider
Journal:  Biophys J       Date:  2009-04-22       Impact factor: 4.033

3.  Towards the total chemical synthesis of integral membrane proteins: a general method for the synthesis of hydrophobic peptide-thioester building blocks.

Authors:  Erik C B Johnson; Stephen B H Kent
Journal:  Tetrahedron Lett       Date:  2007-03-05       Impact factor: 2.415

4.  Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents.

Authors:  Christian Klammt; Marilyn H Perrin; Innokentiy Maslennikov; Ludovic Renault; Martin Krupa; Witek Kwiatkowski; Henning Stahlberg; Wylie Vale; Senyon Choe
Journal:  Protein Sci       Date:  2011-05-03       Impact factor: 6.725

5.  Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli.

Authors:  Pascal Egloff; Matthias Hillenbrand; Christoph Klenk; Alexander Batyuk; Philipp Heine; Stefanie Balada; Karola M Schlinkmann; Daniel J Scott; Marco Schütz; Andreas Plückthun
Journal:  Proc Natl Acad Sci U S A       Date:  2014-01-22       Impact factor: 11.205

6.  High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences.

Authors:  Jonas Lee; Sung-Hou Kim
Journal:  Protein Expr Purif       Date:  2008-09-14       Impact factor: 1.650

7.  Mapping the membrane-aqueous border for the voltage-sensing domain of a potassium channel.

Authors:  Edward J Neale; Honglin Rong; Christopher J Cockcroft; Asipu Sivaprasadarao
Journal:  J Biol Chem       Date:  2007-10-19       Impact factor: 5.157

8.  Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography-electrospray ionization-mass spectrometry.

Authors:  Jenny T C Ho; Jim F White; Reinhard Grisshammer; Sonja Hess
Journal:  Anal Biochem       Date:  2007-12-27       Impact factor: 3.365

9.  Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses.

Authors:  Liselotte Kaiser; Johanna Graveland-Bikker; Dirk Steuerwald; Mélanie Vanberghem; Kara Herlihy; Shuguang Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-07       Impact factor: 11.205

10.  High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1.

Authors:  Hui Ren; Daoyong Yu; Baosheng Ge; Brian Cook; Zhinan Xu; Shuguang Zhang
Journal:  PLoS One       Date:  2009-02-18       Impact factor: 3.240
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