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Literature DB >> 16199508

Conformational changes involved in MscL channel gating measured using FRET spectroscopy.

Ben Corry¹, Paul Rigby, Zhen-Wei Liu, Boris Martinac.

Abstract

We demonstrate that fluorescence resonance energy transfer spectroscopy is a powerful tool for in situ structural analysis of multimeric membrane proteins by measuring the conformational changes involved in gating the mechanosensitive ion channel of large conductance. Ensemble analysis is used to analyze the intensity of light emitted by AlexaFluor-labeled cysteine mutants reconstituted into artificial liposomes before and after acceptor photobleaching. The diameter of the protein is found to increase by 16 A upon channel activation.

Entities: Chemical Gene

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Year: 2005 PMID： 16199508 PMCID： PMC1367003 DOI： 10.1529/biophysj.105.072009

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

10 in total

Review 1. Molecular basis of mechanotransduction in living cells.

Authors: O P Hamill; B Martinac
Journal: Physiol Rev Date: 2001-04 Impact factor: 37.312

2. Open channel structure of MscL and the gating mechanism of mechanosensitive channels.

Authors: Eduardo Perozo; D Marien Cortes; Pornthep Sompornpisut; Anna Kloda; Boris Martinac
Journal: Nature Date: 2002-08-29 Impact factor: 49.962

3. Lighting up single ion channels.

Authors: Paul R Selvin
Journal: Biophys J Date: 2003-01 Impact factor: 4.033

Review 4. Mechanosensitive ion channels: molecules of mechanotransduction.

Authors: Boris Martinac
Journal: J Cell Sci Date: 2004-05-15 Impact factor: 5.285

5. A flexible approach to the calculation of resonance energy transfer efficiency between multiple donors and acceptors in complex geometries.

Authors: Ben Corry; Dylan Jayatilaka; Paul Rigby
Journal: Biophys J Date: 2005-09-30 Impact factor: 4.033

6. Purification and functional reconstitution of the recombinant large mechanosensitive ion channel (MscL) of Escherichia coli.

Authors: C C Häse; A C Le Dain; B Martinac
Journal: J Biol Chem Date: 1995-08-04 Impact factor: 5.157

7. Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.

Authors: G Chang; R H Spencer; A T Lee; M T Barclay; D C Rees
Journal: Science Date: 1998-12-18 Impact factor: 47.728

8. The structure of the potassium channel: molecular basis of K+ conduction and selectivity.

Authors: D A Doyle; J Morais Cabral; R A Pfuetzner; A Kuo; J M Gulbis; S L Cohen; B T Chait; R MacKinnon
Journal: Science Date: 1998-04-03 Impact factor: 47.728

9. Physical principles underlying the transduction of bilayer deformation forces during mechanosensitive channel gating.

Authors: Eduardo Perozo; Anna Kloda; D Marien Cortes; Boris Martinac
Journal: Nat Struct Biol Date: 2002-09

10. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity.

Authors: N Levina; S Tötemeyer; N R Stokes; P Louis; M A Jones; I R Booth
Journal: EMBO J Date: 1999-04-01 Impact factor: 11.598

10 in total

25 in total

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Journal: Eur Biophys J Date: 2015-08-02 Impact factor: 1.733

2. Molecular dynamics study of MscL interactions with a curved lipid bilayer.

Authors: Grischa R Meyer; Justin Gullingsrud; Klaus Schulten; Boris Martinac
Journal: Biophys J Date: 2006-06-02 Impact factor: 4.033

3. A flexible approach to the calculation of resonance energy transfer efficiency between multiple donors and acceptors in complex geometries.

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Journal: Biophys J Date: 2005-09-30 Impact factor: 4.033

Review 4. Ion channels in microbes.

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Journal: Physiol Rev Date: 2008-10 Impact factor: 37.312

5. Bilayer-mediated clustering and functional interaction of MscL channels.

Authors: Stephan L Grage; Asbed M Keleshian; Tamta Turdzeladze; Andrew R Battle; Wee C Tay; Roland P May; Stephen A Holt; Sonia Antoranz Contera; Michael Haertlein; Martine Moulin; Prithwish Pal; Paul R Rohde; V Trevor Forsyth; Anthony Watts; Kerwyn Casey Huang; Anne S Ulrich; Boris Martinac
Journal: Biophys J Date: 2011-03-02 Impact factor: 4.033