Literature DB >> 1617301

Identification of novel steroid-response elements.

Z Nawaz1, M J Tsai, D P McDonnell, B W O'Malley.   

Abstract

A rapid method for defining novel steroid-responsive elements has been developed. Large libraries of degenerate oligonucleotides were analyzed using a yeast-based screen to identify estrogen-responsive DNA sequences. From a library of 40,000 recombinants, seven estrogen-responsive clones were identified. When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE. One surprising result was the presence of the two half sites as direct repeats in some of the clones. This implies that in vivo estrogen receptor can bind and transactivate yeast genes through response elements in which the two half sites align as direct repeats. This protocol requires no purified protein and specifically selects for functional response elements. It has a wide application in the study of any transcription factor/DNA interaction.

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Year:  1992        PMID： 1617301      PMCID： PMC6057364     

Source DB:  PubMed          Journal:  Gene Expr        ISSN： 1052-2166


  31 in total

1.  Sequence elements in the human osteocalcin gene confer basal activation and inducible response to hormonal vitamin D3.

Authors:  S A Kerner; R A Scott; J W Pike
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

2.  Cloning of random-sequence oligodeoxynucleotides.

Authors:  A R Oliphant; A L Nussbaum; K Struhl
Journal:  Gene       Date:  1986       Impact factor: 3.688

3.  Molecular interactions of steroid hormone receptor with its enhancer element: evidence for receptor dimer formation.

Authors:  S Y Tsai; J Carlstedt-Duke; N L Weigel; K Dahlman; J A Gustafsson; M J Tsai; B W O'Malley
Journal:  Cell       Date:  1988-10-21       Impact factor: 41.582

4.  Oestrogen and glucocorticoid responsive elements are closely related but distinct.

Authors:  G Klock; U Strähle; G Schütz
Journal:  Nature       Date:  1987 Oct 22-28       Impact factor: 49.962

5.  A DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression.

Authors:  U Strähle; G Klock; G Schütz
Journal:  Proc Natl Acad Sci U S A       Date:  1987-11       Impact factor: 11.205

6.  A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor.

Authors:  L Klein-Hitpass; G U Ryffel; E Heitlinger; A C Cato
Journal:  Nucleic Acids Res       Date:  1988-01-25       Impact factor: 16.971

7.  A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.

Authors:  C S Hoffman; F Winston
Journal:  Gene       Date:  1987       Impact factor: 3.688

8.  Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.

Authors:  P Walker; J E Germond; M Brown-Luedi; F Givel; W Wahli
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

9.  Variant human breast tumor estrogen receptor with constitutive transcriptional activity.

Authors:  S A Fuqua; S D Fitzgerald; G C Chamness; A K Tandon; D P McDonnell; Z Nawaz; B W O'Malley; W L McGuire
Journal:  Cancer Res       Date:  1991-01-01       Impact factor: 12.701

Review 10.  The steroid and thyroid hormone receptor superfamily.

Authors:  R M Evans
Journal:  Science       Date:  1988-05-13       Impact factor: 47.728
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  5 in total

Review 1.  Estrogen receptor interaction with estrogen response elements.

Authors:  C M Klinge
Journal:  Nucleic Acids Res       Date:  2001-07-15       Impact factor: 16.971

Review 2.  Importance of sex to pain and its amelioration; relevance of spinal estrogens and its membrane receptors.

Authors:  Alan R Gintzler; Nai-Jiang Liu
Journal:  Front Neuroendocrinol       Date:  2012-10-02       Impact factor: 8.606

3.  The yeast SIN3 gene product negatively regulates the activity of the human progesterone receptor and positively regulates the activities of GAL4 and the HAP1 activator.

Authors:  Z Nawaz; C Baniahmad; T P Burris; D J Stillman; B W O'Malley; M J Tsai
Journal:  Mol Gen Genet       Date:  1994-12-15

4.  Functional analysis of mouse Hoxa-7 in Saccharomyces cerevisiae: sequences outside the homeodomain base contact zone influence binding and activation.

Authors:  M K Gross; P Gruss
Journal:  Mol Cell Biol       Date:  1994-01       Impact factor: 4.272

5.  A nuclear hormone receptor-associated protein that inhibits transactivation by the thyroid hormone and retinoic acid receptors.

Authors:  T P Burris; Z Nawaz; M J Tsai; B W O'Malley
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-10       Impact factor: 11.205
  5 in total

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