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Literature DB >> 16145158

Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.

Hanna Hartmann¹, Henrik Stender, Andrea Schäfer, Ingo B Autenrieth, Volkhard A J Kempf.

Abstract

Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.

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Year: 2005 PMID： 16145158 PMCID： PMC1234125 DOI： 10.1128/JCM.43.9.4855-4857.2005

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures.

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2. Rapid identification of pathogens in blood.

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4. Rapid detection and identification of pathogens in blood cultures by fluorescence in situ hybridization and flow cytometry.

Authors: Volkhard A J Kempf; Tanja Mändle; Ulrike Schumacher; Andrea Schäfer; Ingo B Autenrieth
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Review 5. Septic shock.

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6. Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

Authors: Kenneth Oliveira; Gary W Procop; Deborah Wilson; James Coull; Henrik Stender
Journal: J Clin Microbiol Date: 2002-01 Impact factor: 5.948

7. Direct identification of Staphylococcus aureus from positive blood culture bottles.

Authors: Kenneth Oliveira; Stephen M Brecher; Annette Durbin; Daniel S Shapiro; Donald R Schwartz; Paola C De Girolami; Joanna Dakos; Gary W Procop; Deborah Wilson; Chad S Hanna; Gerhard Haase; Heidrun Peltroche-Llacsahuanga; Kimberle C Chapin; Michael C Musgnug; Michael H Levi; Cynthia Shoemaker; Henrik Stender
Journal: J Clin Microbiol Date: 2003-02 Impact factor: 5.948

8. Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH.

Authors: V González; E Padilla; M Giménez; C Vilaplana; A Pérez; G Fernández; M D Quesada; M A Pallarés; V Ausina
Journal: Eur J Clin Microbiol Infect Dis Date: 2004-04-27 Impact factor: 3.267

9. Blood cultures positive for coagulase-negative staphylococci: antisepsis, pseudobacteremia, and therapy of patients.

Authors: D Souvenir; D E Anderson; S Palpant; H Mroch; S Askin; J Anderson; J Claridge; J Eiland; C Malone; M W Garrison; P Watson; D M Campbell
Journal: J Clin Microbiol Date: 1998-07 Impact factor: 5.948

Review 10. An introduction to peptide nucleic acid.

Authors: P E Nielsen; M Egholm
Journal: Curr Issues Mol Biol Date: 1999 Impact factor: 2.081

17 in total

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Authors: C Almeida; N F Azevedo; R M Fernandes; C W Keevil; M J Vieira
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2. Locked nucleic acid and flow cytometry-fluorescence in situ hybridization for the detection of bacterial small noncoding RNAs.

Authors: Kelly L Robertson; Gary J Vora
Journal: Appl Environ Microbiol Date: 2011-11-04 Impact factor: 4.792

3. Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow.

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Journal: J Clin Microbiol Date: 2008-02-20 Impact factor: 5.948

4. Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula.

Authors: C Almeida; N F Azevedo; C Iversen; S Fanning; C W Keevil; M J Vieira
Journal: Appl Environ Microbiol Date: 2009-03-06 Impact factor: 4.792

5. Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles.

Authors: Osman Altun; Mohammed Almuhayawi; Måns Ullberg; Volkan Ozenci
Journal: J Clin Microbiol Date: 2013-10-02 Impact factor: 5.948

6. FISH Variants.

Authors: Nuno M Guimarães; Nuno F Azevedo; Carina Almeida
Journal: Methods Mol Biol Date: 2021

7. An Introduction to Fluorescence in situ Hybridization in Microorganisms.

Authors: Carina Almeida; Nuno F Azevedo
Journal: Methods Mol Biol Date: 2021

Review 8. Developments for improved diagnosis of bacterial bloodstream infections.

Authors: A J M Loonen; P F G Wolffs; C A Bruggeman; A J C van den Brule
Journal: Eur J Clin Microbiol Infect Dis Date: 2014-05-22 Impact factor: 3.267

9. Rapid identification of Staphylococcus aureus and methicillin resistance by flow cytometry using a peptide nucleic acid probe.

Authors: Nabin K Shrestha; Nikole M Scalera; Deborah A Wilson; Byron Brehm-Stecher; Gary W Procop
Journal: J Clin Microbiol Date: 2011-07-27 Impact factor: 5.948

10. Rapid detection of methicillin-resistant staphylococci by real-time PCR directly from positive blood culture bottles.

Authors: S Gröbner; V A J Kempf
Journal: Eur J Clin Microbiol Infect Dis Date: 2007-10 Impact factor: 3.267