Literature DB >> 21795508

Rapid identification of Staphylococcus aureus and methicillin resistance by flow cytometry using a peptide nucleic acid probe.

Nabin K Shrestha1, Nikole M Scalera, Deborah A Wilson, Byron Brehm-Stecher, Gary W Procop.   

Abstract

A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.

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Year:  2011        PMID： 21795508      PMCID： PMC3165574          DOI： 10.1128/JCM.01098-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry.

Authors:  Hanna Hartmann; Henrik Stender; Andrea Schäfer; Ingo B Autenrieth; Volkhard A J Kempf
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

2.  Identification of indicator microorganisms using a standardized PNA FISH method.

Authors:  H Perry-O'Keefe; S Rigby; K Oliveira; D Sørensen; H Stender; J Coull; J J Hyldig-Nielsen
Journal:  J Microbiol Methods       Date:  2001-12       Impact factor: 2.363

3.  Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

Authors:  Kenneth Oliveira; Gary W Procop; Deborah Wilson; James Coull; Henrik Stender
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

4.  Rapid differentiation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by flow cytometry after brief antibiotic exposure.

Authors:  Nabin K Shrestha; Nikole M Scalera; Deborah A Wilson; Gary W Procop
Journal:  J Clin Microbiol       Date:  2011-04-06       Impact factor: 5.948

5.  Successful use of broth microdilution in susceptibility tests for methicillin-resistant (heteroresistant) staphylococci.

Authors:  C Thornsberry; L K McDougal
Journal:  J Clin Microbiol       Date:  1983-11       Impact factor: 5.948
  5 in total
  3 in total

1.  FISH Variants.

Authors:  Nuno M Guimarães; Nuno F Azevedo; Carina Almeida
Journal:  Methods Mol Biol       Date:  2021

2.  Immuno-flow cytometry for the rapid identification of Staphylococcus aureus and the detection of methicillin resistance.

Authors:  N K Shrestha; D A Wilson; N M Scalera; A Oppedahl; G W Procop
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-12-27       Impact factor: 3.267

Review 3.  Flow-FISH as a Tool for Studying Bacteria, Fungi and Viruses.

Authors:  Julian J Freen-van Heeren
Journal:  BioTech (Basel)       Date:  2021-10-11
  3 in total

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