Literature DB >> 16137685

Biochemical characterization of the HydE and HydG iron-only hydrogenase maturation enzymes from Thermatoga maritima.

Jon K Rubach1, Xavier Brazzolotto, Jacques Gaillard, Marc Fontecave.   

Abstract

Fe-only hydrogenases contain a di-iron active site complex, in which the two Fe atoms have carbon monoxide and cyanide ligands and are linked together by a putative di(thiomethyl)amine molecule. We have cloned, purified and characterized the HydE and HydG proteins, thought to be involved in the biosynthesis of this peculiar metal site, from the thermophilic organism Thermotoga maritima. The HydE protein anaerobically reconstituted with iron and sulfide binds two [4Fe-4S] clusters, as characterized by UV and EPR spectroscopy. The HydG protein binds one [4Fe-4S] cluster, and probably an additional one. Both enzymes are able to reductively cleave S-adenosylmethionine (SAM) when reduced by dithionite, confirming that they are Radical-SAM enzymes.

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Year:  2005        PMID： 16137685     DOI： 10.1016/j.febslet.2005.07.092

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  48 in total

1.  Crystal structure of HydF scaffold protein provides insights into [FeFe]-hydrogenase maturation.

Authors:  Laura Cendron; Paola Berto; Sarah D'Adamo; Francesca Vallese; Chiara Govoni; Matthew C Posewitz; Giorgio M Giacometti; Paola Costantini; Giuseppe Zanotti
Journal:  J Biol Chem       Date:  2011-11-04       Impact factor: 5.157

Review 2.  Structure-function relationships in [FeFe]-hydrogenase active site maturation.

Authors:  Yvain Nicolet; Juan C Fontecilla-Camps
Journal:  J Biol Chem       Date:  2012-03-02       Impact factor: 5.157

Review 3.  Control of radical chemistry in the AdoMet radical enzymes.

Authors:  Kaitlin S Duschene; Susan E Veneziano; Sunshine C Silver; Joan B Broderick
Journal:  Curr Opin Chem Biol       Date:  2009-03-09       Impact factor: 8.822

4.  Identification and characterization of a novel member of the radical AdoMet enzyme superfamily and implications for the biosynthesis of the Hmd hydrogenase active site cofactor.

Authors:  Shawn E McGlynn; Eric S Boyd; Eric M Shepard; Rachel K Lange; Robin Gerlach; Joan B Broderick; John W Peters
Journal:  J Bacteriol       Date:  2009-11-06       Impact factor: 3.490

5.  The final steps of [FeFe]-hydrogenase maturation.

Authors:  Oliver Lampret; Julian Esselborn; Rieke Haas; Andreas Rutz; Rosalind L Booth; Leonie Kertess; Florian Wittkamp; Clare F Megarity; Fraser A Armstrong; Martin Winkler; Thomas Happe
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-23       Impact factor: 11.205

Review 6.  Emerging themes in radical SAM chemistry.

Authors:  Krista A Shisler; Joan B Broderick
Journal:  Curr Opin Struct Biol       Date:  2012-11-08       Impact factor: 6.809

7.  A cell-free microtiter plate screen for improved [FeFe] hydrogenases.

Authors:  James A Stapleton; James R Swartz
Journal:  PLoS One       Date:  2010-05-10       Impact factor: 3.240

8.  Mechanistic and functional versatility of radical SAM enzymes.

Authors:  Squire J Booker; Tyler L Grove
Journal:  F1000 Biol Rep       Date:  2010-07-14

9.  High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli.

Authors:  Jon M Kuchenreuther; Celestine S Grady-Smith; Alyssa S Bingham; Simon J George; Stephen P Cramer; James R Swartz
Journal:  PLoS One       Date:  2010-11-24       Impact factor: 3.240

10.  Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation.

Authors:  Jon M Kuchenreuther; James A Stapleton; James R Swartz
Journal:  PLoS One       Date:  2009-10-26       Impact factor: 3.240
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