Literature DB >> 16122230

Fluorous affinity purification of oligonucleotides.

William H Pearson1, David A Berry, Patrick Stoy, Kee-Yong Jung, Anthony D Sercel.   

Abstract

[structure: see text] Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries (typically 70-100%), and the ability to purify long oligonucleotides (e.g., 50-100-mers).

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Year:  2005        PMID： 16122230     DOI： 10.1021/jo050795y

Source DB:  PubMed          Journal:  J Org Chem        ISSN： 0022-3263            Impact factor:   4.354


  11 in total

1.  Tritylation of Alcohols under Mild Conditions without Using Silver Salts.

Authors:  Shahien Shahsavari; Jinsen Chen; Travis Wigstrom; James Gooding; Alexander Gauronskas; Shiyue Fang
Journal:  Tetrahedron Lett       Date:  2016-07-18       Impact factor: 2.415

2.  Synthetic applications of fluorous solid-phase extraction (F-SPE).

Authors:  Wei Zhang; Dennis P Curran
Journal:  Tetrahedron       Date:  2006-12-18       Impact factor: 2.457

3.  A High-Throughput Process for the Solid-Phase Purification of Synthetic DNA Sequences.

Authors:  Andrzej Grajkowski; Jacek Cieślak; Serge L Beaucage
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2017-06-19

4.  Fluorous mixture synthesis of two libraries with hydantoin-, and benzodiazepinedione-fused heterocyclic scaffolds.

Authors:  Wei Zhang; Yimin Lu; Christine Hiu-Tung Chen; Lu Zeng; Daniel B Kassel
Journal:  J Comb Chem       Date:  2006 Sep-Oct

5.  A fluorous phosphate protecting group with applications to carbohydrate synthesis.

Authors:  Lin Liu; Nicola L B Pohl
Journal:  Org Lett       Date:  2011-03-08       Impact factor: 6.005

6.  Sequence-specific purification of DNA oligomers in hydrophobic interaction chromatography using peptide nucleic acid amphiphiles: extended dynamic range.

Authors:  Jeffrey M Savard; James W Schneider
Journal:  Biotechnol Bioeng       Date:  2007-06-01       Impact factor: 4.530

7.  A New Paradigm for Protein Design and Biological Self-Assembly.

Authors:  Gizem Akçay; Krishna Kumar
Journal:  J Fluor Chem       Date:  2009-12-01       Impact factor: 2.050

8.  Highly parallel oligonucleotide purification and functionalization using reversible chemistry.

Authors:  Kerri T York; Ryan C Smith; Rob Yang; Peter C Melnyk; Melissa M Wiley; Casey M Turk; Mostafa Ronaghi; Kevin L Gunderson; Frank J Steemers
Journal:  Nucleic Acids Res       Date:  2011-10-29       Impact factor: 16.971

9.  Directed formation of DNA nanoarrays through orthogonal self-assembly.

Authors:  Jonathan R Burns; Jurgita Zekonyte; Giuliano Siligardi; Rohanah Hussain; Eugen Stulz
Journal:  Molecules       Date:  2011-06-15       Impact factor: 4.411

10.  High-Throughput Amenable MALDI-MS Detection of RNA and DNA with On-Surface Analyte Enrichment Using Fluorous Partitioning.

Authors:  Cole Emanuelson; Nicholas Ankenbruck; Alexander Deiters; Marvin S Yu
Journal:  SLAS Discov       Date:  2020-09-29       Impact factor: 3.341
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