| Literature DB >> 16099169 |
Chen Ren1, Liwen Zhang, Michael A Freitas, Kalpana Ghoshal, Mark R Parthun, Samson T Jacob.
Abstract
The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.Entities:
Mesh：
Substances：
Year: 2005 PMID： 16099169 PMCID： PMC2749475 DOI： 10.1016/j.jasms.2005.06.001
Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.109