AIM: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, SNU-C4. METHODS: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. RESULTS: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 microg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 microg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 microg/mL) compared with control. CONCLUSION: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.
AIM: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on humancolorectal cancer cell line, SNU-C4. METHODS:Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were performed. RESULTS: In this study, cytotoxic effect of OPC on SNU-C4 cells appeared in a dose-dependent manner. OPC treatment (100 microg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 microg/mL) increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 microg/mL) compared with control. CONCLUSION: These data indicate that OPC caused cell death by apoptosis through caspase pathways on humancolorectal cancer cell line, SNU-C4.
Authors: Stéphanie Carnésecchi; Yann Schneider; Sheryl A Lazarus; David Coehlo; Francine Gossé; Francis Raul Journal: Cancer Lett Date: 2002-01-25 Impact factor: 8.679
Authors: James B Thoden; Erika A Taylor Ringia; James B Garrett; John A Gerlt; Hazel M Holden; Ivan Rayment Journal: Biochemistry Date: 2004-05-18 Impact factor: 3.162
Authors: Bin Zhou; Yvette Alania; Mariana Reis; Rasika S Phansalkar; Joo-Won Nam; James B McAlpine; Shao-Nong Chen; Ana K Bedran-Russo; Guido F Pauli Journal: J Org Chem Date: 2020-06-17 Impact factor: 4.354
Authors: Qing-Wei Zheng; Shu-Xian Gao; Jie Lv; Deng-Yu Chen; Jie Chen; Hui-Hui Li; Jun-Chang Guan Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2018-04-20
Authors: Bin Zhou; Yvette Alania; Mariana C Reis; James B McAlpine; Ana K Bedran-Russo; Guido F Pauli; Shao-Nong Chen Journal: Org Lett Date: 2020-07-08 Impact factor: 6.005
Authors: José M Pérez-Ortiz; Luis F Alguacil; Elisabet Salas; Isidro Hermosín-Gutiérrez; Sergio Gómez-Alonso; Carmen González-Martín Journal: Food Sci Nutr Date: 2019-08-02 Impact factor: 2.863