Literature DB >> 16089439

In vivo reporter labeling of proteins via metabolic delivery of coenzyme A analogues.

Kristine M Clarke1, Andrew C Mercer, James J La Clair, Michael D Burkart.   

Abstract

We demonstrate site-specific reporter labeling of proteins within live cells. By accessing the coenzyme A (CoA) metabolic pathway with a cell-permeable pantetheine analogue, we deliver CoA-bound reporter molecules for post-translational protein modification in vivo. These methods may be applied to natural product pathway manipulation as well as applications in conventional molecular and cellular biology.

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Year:  2005        PMID： 16089439     DOI： 10.1021/ja052911k

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  33 in total

1.  Inhibitors of polyhydroxyalkanoate (PHA) synthases: synthesis, molecular docking, and implications.

Authors:  Wei Zhang; Chao Chen; Ruikai Cao; Leila Maurmann; Ping Li
Journal:  Chembiochem       Date:  2014-11-13       Impact factor: 3.164

2.  Inhibitors of aminoglycoside resistance activated in cells.

Authors:  Kenward Vong; Ingrid S Tam; Xuxu Yan; Karine Auclair
Journal:  ACS Chem Biol       Date:  2012-01-18       Impact factor: 5.100

3.  An orthogonal purification strategy for isolating crosslinked domains of modular synthases.

Authors:  Robert W Haushalter; Andrew S Worthington; Gene H Hur; Michael D Burkart
Journal:  Bioorg Med Chem Lett       Date:  2008-01-11       Impact factor: 2.823

Review 4.  Enzymatic labeling of proteins: techniques and approaches.

Authors:  Mohammad Rashidian; Jonathan K Dozier; Mark D Distefano
Journal:  Bioconjug Chem       Date:  2013-08-21       Impact factor: 4.774

Review 5.  The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life.

Authors:  Joris Beld; Eva C Sonnenschein; Christopher R Vickery; Joseph P Noel; Michael D Burkart
Journal:  Nat Prod Rep       Date:  2014-01       Impact factor: 13.423

6.  An eight residue fragment of an acyl carrier protein suffices for post-translational introduction of fluorescent pantetheinyl arms in protein modification in vitro and in vivo.

Authors:  Zhe Zhou; Alexander Koglin; Yu Wang; Andrew P McMahon; Christopher T Walsh
Journal:  J Am Chem Soc       Date:  2008-07-02       Impact factor: 15.419

7.  Synthesis of 4'-aminopantetheine and derivatives to probe aminoglycoside N-6'-acetyltransferase.

Authors:  Xuxu Yan; T Olukayode Akinnusi; Aaron T Larsen; Karine Auclair
Journal:  Org Biomol Chem       Date:  2011-01-12       Impact factor: 3.876

8.  Stereochemical modification of geminal dialkyl substituents on pantothenamides alters antimicrobial activity.

Authors:  Annabelle Hoegl; Hamed Darabi; Elisa Tran; Emelia Awuah; Eleanor S C Kerdo; Eric Habib; Kevin J Saliba; Karine Auclair
Journal:  Bioorg Med Chem Lett       Date:  2014-06-18       Impact factor: 2.823

9.  Exploring structural motifs necessary for substrate binding in the active site of Escherichia coli pantothenate kinase.

Authors:  Emelia Awuah; Eric Ma; Annabelle Hoegl; Kenward Vong; Eric Habib; Karine Auclair
Journal:  Bioorg Med Chem       Date:  2014-04-24       Impact factor: 3.641

10.  An orthogonal active site identification system (OASIS) for proteomic profiling of natural product biosynthesis.

Authors:  Jordan L Meier; Sherry Niessen; Heather S Hoover; Timothy L Foley; Benjamin F Cravatt; Michael D Burkart
Journal:  ACS Chem Biol       Date:  2009-11-20       Impact factor: 5.100
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