| Literature DB >> 16076961 |
Hanako Ikeda1, Fumitaka Osakada, Kiichi Watanabe, Kenji Mizuseki, Tomoko Haraguchi, Hiroyuki Miyoshi, Daisuke Kamiya, Yoshihito Honda, Noriaki Sasai, Nagahisa Yoshimura, Masayo Takahashi, Yoshiki Sasai.
Abstract
We report directed differentiaion of retinal precursors in vitro from mouse ES cells. Six3+ rostral brain progenitors are generated by culturing ES cells under serum-free suspension conditions (SFEB culture) in the presence of Wnt and Nodal antagonists (Dkk1 and LeftyA), and subsequently steered to differentiate into Rx+ cells (16%) by treatment with activin and serum. Consistent with the characteristics of early neural retinal precursors, the induced Rx+ cells coexpress Pax6 and the mitotic marker Ki67, but not Nestin. The ES cell-derived precursors efficiently generate cells with the photoreceptor phenotype (rhodopsin+, recoverin+) when cocultured with embryonic retinal cells. Furthermore, organotypic culture studies demonstrate the selective integration and survival of ES cell-derived cells with the photoreceptor phenotype (marker expression and morphology) in the outer nuclear layer of the retina. Taken together, ES cells treated with SFEB/Dkk1/LeftyA/serum/activin generate neural retinal precursors, which have the competence of photoreceptor differentiation.Entities:
Mesh：
Substances：
Year: 2005 PMID： 16076961 PMCID： PMC1183536 DOI： 10.1073/pnas.0500010102
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205