Hongxi Wu1, Jia Li1, Xinbang Mao1, Guodong Li1, Lin Xie1, Zhipeng You2. 1. Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, 330000, China. 2. Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, 1 Minde Road, Nanchang, 330000, China. yz8014829puxianta@yeah.net.
Abstract
AIM OF STUDY: To evaluate the feasibility of transplantation of embryonic stem cell (ESC)-derived retinal cells in the treatment of retinal degeneration. MATERIALS AND METHODS: Rat ESCs were isolated and induced into retinal progenitor cells (RPCs) in vitro, which were subsequently induced into retinal pigment epithelium cells (RPEs) and photoreceptors (PRCs). All cells were identified by Western blot detection of their specific markers. RPEs and PRCs were, respectively, injected into the retina of Royal College of Surgeons (RCSs) rats. Control group was injected with PBS. Post-transplantation visual function was determined by electroretinography (ERG). The histology of the whole eye was compared by H&E staining. RESULTS: RPEs and PRCs were successfully derived from rat ESCs through the two-step differentiation as indicated by the presence of ESC- (Oct-3/4, Nanog, TRA-1-60 and TRA-1-81), RPC- (Rx, Mitf, Pax6 and Chx10), RPE- (RPE65 and keratin) and PRC-specific markers (blue opsin, red/green opsin, recoverin and rhodopsin) in Western blot. The amplitude of ERG a- and b-wave in RPE- and PRC-transplanted groups at week 2 and 10 after transplantation was markedly higher compared with PBS controls. Retinal injury and vascular stress response was not detected in any of the RCS rats after transplantation. CONCLUSION: The developed stepwise protocol can derive retinal cells from ESCs. Transplantation of these retinal cells can restore visual function of RCS rats. Our study provides evidence for potential clinical application of ESC-based cell therapy for retinal degeneration.
AIM OF STUDY: To evaluate the feasibility of transplantation of embryonic stem cell (ESC)-derived retinal cells in the treatment of retinal degeneration. MATERIALS AND METHODS:Rat ESCs were isolated and induced into retinal progenitor cells (RPCs) in vitro, which were subsequently induced into retinal pigment epithelium cells (RPEs) and photoreceptors (PRCs). All cells were identified by Western blot detection of their specific markers. RPEs and PRCs were, respectively, injected into the retina of Royal College of Surgeons (RCSs) rats. Control group was injected with PBS. Post-transplantation visual function was determined by electroretinography (ERG). The histology of the whole eye was compared by H&E staining. RESULTS: RPEs and PRCs were successfully derived from rat ESCs through the two-step differentiation as indicated by the presence of ESC- (Oct-3/4, Nanog, TRA-1-60 and TRA-1-81), RPC- (Rx, Mitf, Pax6 and Chx10), RPE- (RPE65 and keratin) and PRC-specific markers (blue opsin, red/green opsin, recoverin and rhodopsin) in Western blot. The amplitude of ERG a- and b-wave in RPE- and PRC-transplanted groups at week 2 and 10 after transplantation was markedly higher compared with PBS controls. Retinal injury and vascular stress response was not detected in any of the RCS rats after transplantation. CONCLUSION: The developed stepwise protocol can derive retinal cells from ESCs. Transplantation of these retinal cells can restore visual function of RCS rats. Our study provides evidence for potential clinical application of ESC-based cell therapy for retinal degeneration.
Authors: Jianmin Zhang; Russell J Taylor; Anna La Torre; Matthew S Wilken; Kristen E Cox; Thomas A Reh; Monica L Vetter Journal: Dev Biol Date: 2015-05-16 Impact factor: 3.582
Authors: P M D'Cruz; D Yasumura; J Weir; M T Matthes; H Abderrahim; M M LaVail; D Vollrath Journal: Hum Mol Genet Date: 2000-03-01 Impact factor: 6.150
Authors: Sabine Aisenbrey; Bart A Lafaut; Peter Szurman; Ralph-Dieter Hilgers; Peter Esser; Peter Walter; Karl U Bartz-Schmidt; Gabriele Thumann Journal: Arch Ophthalmol Date: 2006-02
Authors: Deepthi S Rajendran Nair; Magdalene J Seiler; Kahini H Patel; Vinoy Thomas; Juan Carlos Martinez Camarillo; Mark S Humayun; Biju B Thomas Journal: Appl Sci (Basel) Date: 2021-02-28 Impact factor: 2.679
Authors: Céline Koster; Kimberley E Wever; Philip E Wagstaff; Koen T van den Hirk; Carlijn R Hooijmans; Arthur A Bergen Journal: Int J Mol Sci Date: 2020-04-14 Impact factor: 5.923