| Literature DB >> 16043309 |
Jukka Kurola1, Mirja Salkinoja-Salonen, Tuula Aarnio, Jenni Hultman, Martin Romantschuk.
Abstract
Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4-9 x 10(5) cellsg(-1) of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation (0.05-0.28 microg NO2(-)-N g(-1) of dry soil h(-1)) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments.Entities:
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Year: 2005 PMID： 16043309 DOI： 10.1016/j.femsle.2005.06.057
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742