| Literature DB >> 16028888 |
Ivan Rasnik1, Sean A McKinney, Taekjip Ha.
Abstract
Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.Mesh:
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Year: 2005 PMID: 16028888 DOI: 10.1021/ar040138c
Source DB: PubMed Journal: Acc Chem Res ISSN: 0001-4842 Impact factor: 22.384