Literature DB >> 16028062

Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes.

Jingxue Wang1, Zhenlang Chen, Jianzhong Du, Yi Sun, Aihua Liang.   

Abstract

Transgenic plants with introduced pest-resistant gene offer an efficient alternative insect control. The novel insect-resistant gene combination, chitinase(chi) and BmkIT(Bmk), containing an insect-specific chitinase gene and a scorpion insect toxin gene was introduced into Brassica napus cultivar via Agrobacterium-mediated transformation. Fifty-seven regenerated plantlets with kanamycin-resistance were obtained. Transgenic plants were verified by Southern blot analysis. Enzyme-linked immunosorbent assay (ELISA) and bioassay of artificial inoculation with diamondback moth (Plutella maculipenis) (DBM) larvae indicated that some of the transgenic plants were high-level expression for both chitinase and scorpion toxin proteins and performed high resistance against the tested pest infestation. The genetic analysis of T(1) progeny confirmed that the inheritance of introduced genes followed the Mendelian rules.

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Year:  2005        PMID： 16028062     DOI： 10.1007/s00299-005-0967-3

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  13 in total

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  10 in total

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Journal:  J Appl Genet       Date:  2016-02-10       Impact factor: 3.240

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7.  Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil α-amylase.

Authors:  Guilherme Souza Prado; Pingdwende Kader Aziz Bamogo; Joel Antônio Cordeiro de Abreu; François-Xavier Gillet; Vanessa Olinto Dos Santos; Maria Cristina Mattar Silva; Jean-Paul Brizard; Marcelo Porto Bemquerer; Martine Bangratz; Christophe Brugidou; Drissa Sérémé; Maria Fatima Grossi-de-Sa; Séverine Lacombe
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8.  Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.

Authors:  Xiuzi Tianpei; Yingguo Zhu; Shaoqing Li
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Authors:  Fang Liu; Pandi Wang; Xiaojuan Xiong; Ping Fu; Hongfei Gao; Xinhua Ding; Gang Wu
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Authors:  Nian Wang; Feng Li; Biyun Chen; Kun Xu; Guixin Yan; Jiangwei Qiao; Jun Li; Guizhen Gao; Ian Bancroft; Jingling Meng; Graham J King; Xiaoming Wu
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  10 in total

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