Literature DB >> 16012830

A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA.

P Grubwieser1, R Mühlmann, B Berger, H Niederstätter, M Pavlic, W Parson.   

Abstract

A multiplex PCR was designed for the loci D2S1338, D16S539, D18S51, TH01 and FGA using redesigned primers in order to reduce the lengths of the amplification products compared to the designs used in commercially available multiplex PCR kits, also including amelogenin. The new PCR primers were used to amplify highly degraded DNA from casework samples, which had shown no or only poor results for these loci in previous analyses with standard primer sets. The application of the new miniSTR-multiplex resulted in an increased overall typing success rate for degraded DNA samples. In a concordance study between the conventional and the newly designed primers, no genotype differences were revealed in 124 randomly selected individuals.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16012830     DOI： 10.1007/s00414-005-0013-6

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  17 in total

1.  Less is more--length reduction of STR amplicons using redesigned primers.

Authors:  P Wiegand; M Kleiber
Journal:  Int J Legal Med       Date:  2001       Impact factor: 2.686

2.  STR typing of human telogen hairs--a new approach.

Authors:  A Hellmann; U Rohleder; H Schmitter; M Wittig
Journal:  Int J Legal Med       Date:  2001       Impact factor: 2.686

3.  Criminal DNA databases: the European situation.

Authors:  P M Schneider; P D Martin
Journal:  Forensic Sci Int       Date:  2001-06-15       Impact factor: 2.395

4.  The development of reduced size STR amplicons as tools for analysis of degraded DNA.

Authors:  John M Butler; Yin Shen; Bruce R McCord
Journal:  J Forensic Sci       Date:  2003-09       Impact factor: 1.832

5.  STR analysis of artificially degraded DNA-results of a collaborative European exercise.

Authors:  Peter M Schneider; Klaus Bender; Wolfgang R Mayr; Walther Parson; Bernadette Hoste; Ronny Decorte; Jan Cordonnier; Daniel Vanek; Niels Morling; Matti Karjalainen; C Marie-Paule Carlotti; Myriam Sabatier; Carsten Hohoff; Hermann Schmitter; Werner Pflug; Rainer Wenzel; Dieter Patzelt; Rüdiger Lessig; Peter Dobrowolski; Geraldine O'Donnell; Luciano Garafano; Marina Dobosz; Peter De Knijff; Bente Mevag; Ryszard Pawlowski; Leonor Gusmão; Maria Conceicao Vide; Antonio Alonso Alonso; Oscar García Fernández; Pilar Sanz Nicolás; Ann Kihlgreen; Walter Bär; Verena Meier; Anne Teyssier; Raphael Coquoz; Conxita Brandt; Ursula Germann; Peter Gill; Justine Hallett; Matthew Greenhalgh
Journal:  Forensic Sci Int       Date:  2004-01-28       Impact factor: 2.395

6.  Influence of soil storage and exposure period on DNA recovery from teeth.

Authors:  H Pfeiffer; J Hühne; B Seitz; B Brinkmann
Journal:  Int J Legal Med       Date:  1999       Impact factor: 2.686

7.  Study on the STR TPOX in an Italian and an Austrian population using two different primer pairs and three different electrophoretic methods.

Authors:  U Ricci; M Klintschar; F Neuhuber; M L Giovannucci Uzielli
Journal:  Int J Legal Med       Date:  1998       Impact factor: 2.686

8.  Effect of environmental factors on PCR-DNA analysis from dental pulp.

Authors:  A Alvarez García; I Muñoz; C Pestoni; M V Lareu; M S Rodríguez-Calvo; A Carracedo
Journal:  Int J Legal Med       Date:  1996       Impact factor: 2.686

9.  Evaluation of new primers for CSF1PO.

Authors:  K Yoshida; K Sekiguchi; K Kasai; H Sato; S Seta; G F Sensabaugh
Journal:  Int J Legal Med       Date:  1997       Impact factor: 2.686

10.  Preparation of degraded human DNA under controlled conditions.

Authors:  Klaus Bender; María José Farfán; Peter M Schneider
Journal:  Forensic Sci Int       Date:  2004-01-28       Impact factor: 2.395
View more
  23 in total

1.  Assessment of DNA degradation and the genotyping success of highly degraded samples.

Authors:  Sheree R Hughes-Stamm; Kevin J Ashton; Angela van Daal
Journal:  Int J Legal Med       Date:  2010-04-24       Impact factor: 2.686

2.  First experiences using the new Powerplex® ESX17 and ESI17 kits in casework analysis and allele frequencies for two different regions in Germany.

Authors:  Micaela Poetsch; Katharina Bayer; Zeynep Ergin; Marco Milbrath; Thorsten Schwark; Nicole von Wurmb-Schwark
Journal:  Int J Legal Med       Date:  2010-06-22       Impact factor: 2.686

3.  Population data on the seven short tandem repeat loci D4S2366, D6S474, D14S608, D19S246, D20S480, D21S226 and D22S689 in a German population.

Authors:  Dorit Becker; Dorit Vogelsang; Werner Brabetz
Journal:  Int J Legal Med       Date:  2005-11-19       Impact factor: 2.686

4.  Y-STR analysis of degraded DNA using reduced-size amplicons.

Authors:  Myung Jin Park; Hwan Young Lee; Ukhee Chung; Seung-Chul Kang; Kyoung-Jin Shin
Journal:  Int J Legal Med       Date:  2006-11-15       Impact factor: 2.686

5.  First successful assay of Y-SNP typing by SNaPshot minisequencing on ancient DNA.

Authors:  C Bouakaze; C Keyser; S Amory; E Crubézy; B Ludes
Journal:  Int J Legal Med       Date:  2007-05-30       Impact factor: 2.686

6.  Accuracy of metric sex analysis of skeletal remains using Fordisc based on a recent skull collection.

Authors:  F Ramsthaler; K Kreutz; M A Verhoff
Journal:  Int J Legal Med       Date:  2007-11       Impact factor: 2.686

7.  Evaluation of miniY-STR multiplex PCR systems for extended 16 Y-STR loci.

Authors:  H Asamura; S Fujimori; M Ota; T Oki; H Fukushima
Journal:  Int J Legal Med       Date:  2007-09-26       Impact factor: 2.686

8.  "GenderPlex" a PCR multiplex for reliable gender determination of degraded human DNA samples and complex gender constellations.

Authors:  Anna Esteve Codina; Harald Niederstätter; Walther Parson
Journal:  Int J Legal Med       Date:  2008-12-17       Impact factor: 2.686

9.  'Mitominis': multiplex PCR analysis of reduced size amplicons for compound sequence analysis of the entire mtDNA control region in highly degraded samples.

Authors:  Cordula Eichmann; Walther Parson
Journal:  Int J Legal Med       Date:  2008-03-28       Impact factor: 2.686

10.  Increasing the discrimination power of forensic STR testing by employing high-performance mass spectrometry, as illustrated in indigenous South African and Central Asian populations.

Authors:  Florian Pitterl; Konrad Schmidt; Gabriela Huber; Bettina Zimmermann; Rhena Delport; Sylvain Amory; Bertrand Ludes; Herbert Oberacher; Walther Parson
Journal:  Int J Legal Med       Date:  2010-01-16       Impact factor: 2.686
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.