Literature DB >> 16000494

Detection of Tropheryma whipplei DNA in clinical specimens by LightCycler real-time PCR.

Lynne M Sloan1, Jon E Rosenblatt, Franklin R Cockerill.   

Abstract

A real-time PCR method using the LightCycler (Roche Applied Science, Indianapolis, IN) was compared to a conventional PCR assay for the detection of Tropheryma whipplei in 321 clinical specimens. The LightCycler method had sensitivity and specificity comparable to the conventional method but required considerably less labor and time (3 h versus 3 to 5 days).

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Year:  2005        PMID： 16000494      PMCID： PMC1169151          DOI： 10.1128/JCM.43.7.3516-3518.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

Review 1.  Whipple's disease and "Tropheryma whippelii".

Authors:  F Dutly; M Altwegg
Journal:  Clin Microbiol Rev       Date:  2001-07       Impact factor: 26.132

2.  Deactivation of macrophages with interleukin-4 is the key to the isolation of Tropheryma whippelii.

Authors:  G Schoedon; D Goldenberger; R Forrer; A Gunz; F Dutly; M Höchli; M Altwegg; A Schaffner
Journal:  J Infect Dis       Date:  1997-09       Impact factor: 5.226

3.  rpoB sequence analysis of cultured Tropheryma whippelii.

Authors:  M Drancourt; A Carlioz; D Raoult
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

4.  Cultivation of the bacillus of Whipple's disease.

Authors:  D Raoult; M L Birg; B La Scola; P E Fournier; M Enea; H Lepidi; V Roux; J C Piette; F Vandenesch; D Vital-Durand; T J Marrie
Journal:  N Engl J Med       Date:  2000-03-02       Impact factor: 91.245

5.  Tropheryma whippelii DNA is rare in the intestinal mucosa of patients without other evidence of Whipple disease.

Authors:  M Maiwald; A von Herbay; D H Persing; P P Mitchell; M F Abdelmalek; J N Thorvilson; D N Fredricks; D A Relman
Journal:  Ann Intern Med       Date:  2001-01-16       Impact factor: 25.391

6.  Antibiotic susceptibility of Tropheryma whipplei in MRC5 cells.

Authors:  Areen Boulos; Jean-Marc Rolain; Didier Raoult
Journal:  Antimicrob Agents Chemother       Date:  2004-03       Impact factor: 5.191
  6 in total
  5 in total

1.  Whipple's DNA is not Whipple's disease.

Authors:  Markus Schneemann; Gabriele Schoedon
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Inhibition controls for qualitative real-time PCR assays: are they necessary for all specimen matrices?

Authors:  S P Buckwalter; L M Sloan; S A Cunningham; M J Espy; J R Uhl; M F Jones; E A Vetter; J Mandrekar; F R Cockerill; B S Pritt; R Patel; N L Wengenack
Journal:  J Clin Microbiol       Date:  2014-04-16       Impact factor: 5.948

3.  PCR-electrospray ionization mass spectrometry for direct detection of pathogens and antimicrobial resistance from heart valves in patients with infective endocarditis.

Authors:  Cassandra L Brinkman; Paschalis Vergidis; James R Uhl; Bobbi S Pritt; Franklin R Cockerill; James M Steckelberg; Larry M Baddour; Joseph J Maleszewski; William D Edwards; Rangarajan Sampath; Robin Patel
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

4.  Validation of an rpoB gene PCR assay for detection of Tropheryma whipplei: 10 years' experience in a National Reference Laboratory.

Authors:  Annette Moter; Dinah Schmiedel; Annett Petrich; Alexandra Wiessner; Judith Kikhney; Thomas Schneider; Verena Moos; Ulf B Göbel; Udo Reischl
Journal:  J Clin Microbiol       Date:  2013-08-21       Impact factor: 5.948

5.  Diagnostic Approach for Classic Compared With Localized Whipple Disease.

Authors:  Nicholas R Crews; Kelly A Cawcutt; Bobbi S Pritt; Robin Patel; Abinash Virk
Journal:  Open Forum Infect Dis       Date:  2018-06-13       Impact factor: 3.835
  5 in total

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