Literature DB >> 23966507

Validation of an rpoB gene PCR assay for detection of Tropheryma whipplei: 10 years' experience in a National Reference Laboratory.

Annette Moter1, Dinah Schmiedel, Annett Petrich, Alexandra Wiessner, Judith Kikhney, Thomas Schneider, Verena Moos, Ulf B Göbel, Udo Reischl.   

Abstract

The performance of a real-time PCR assay targeting the Tropheryma whipplei rpoB gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. The novel rpoB-PCR assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of T. whipplei DNA in clinical specimens.

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Year:  2013        PMID： 23966507      PMCID： PMC3889782          DOI： 10.1128/JCM.01703-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Quantitative detection of Tropheryma whipplei DNA by real-time PCR.

Authors:  Florence Fenollar; Pierre-Edouard Fournier; Didier Raoult; Rene Gérolami; Hubert Lepidi; Claire Poyart
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

2.  Detection of Tropheryma whipplei DNA in clinical specimens by LightCycler real-time PCR.

Authors:  Lynne M Sloan; Jon E Rosenblatt; Franklin R Cockerill
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

3.  daime, a novel image analysis program for microbial ecology and biofilm research.

Authors:  Holger Daims; Sebastian Lücker; Michael Wagner
Journal:  Environ Microbiol       Date:  2006-02       Impact factor: 5.491

4.  Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.

Authors:  D J Lane; B Pace; G J Olsen; D A Stahl; M L Sogin; N R Pace
Journal:  Proc Natl Acad Sci U S A       Date:  1985-10       Impact factor: 11.205

5.  Intravenous ceftriaxone, followed by 12 or three months of oral treatment with trimethoprim-sulfamethoxazole in Whipple's disease.

Authors:  Gerhard E Feurle; Verena Moos; Hendrik Bläker; Christoph Loddenkemper; Annette Moter; Andrea Stroux; Thomas Marth; Thomas Schneider
Journal:  J Infect       Date:  2013-01-03       Impact factor: 6.072

6.  Diagnostic application of a polymerase chain reaction assay for the Whipple's disease bacterium to intestinal biopsies.

Authors:  A von Herbay; H J Ditton; M Maiwald
Journal:  Gastroenterology       Date:  1996-06       Impact factor: 22.682

7.  Detection of three different types of 'Tropheryma whippelii' directly from clinical specimens by sequencing, single-strand conformation polymorphism (SSCP) analysis and type-specific PCR of their 16S-23S ribosomal intergenic spacer region.

Authors:  H P Hinrikson; F Dutly; S Nair; M Altwegg
Journal:  Int J Syst Bacteriol       Date:  1999-10

8.  Cloning and sequencing an unknown gene of Tropheryma whipplei and development of two LightCycler PCR assays.

Authors:  Romana C Maibach; Martin Altwegg
Journal:  Diagn Microbiol Infect Dis       Date:  2003-07       Impact factor: 2.803

9.  Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei.

Authors:  Florence Fenollar; Pierre-Edouard Fournier; Catherine Robert; Didier Raoult
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

10.  False positive PCR detection of Tropheryma whipplei in the saliva of healthy people.

Authors:  Jean-Marc Rolain; Florence Fenollar; Didier Raoult
Journal:  BMC Microbiol       Date:  2007-05-29       Impact factor: 3.605
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  7 in total

1.  Whipple's Disease: Diagnostic Value of rpoB Gene PCR from Peripheral Blood Mononuclear Cells.

Authors:  Kathleen Weigt; Alexandra Wiessner; Annette Moter; Florence Fenollar; Didier Raoult; Kristina Allers; Thomas Schneider; Verena Moos
Journal:  Mol Diagn Ther       Date:  2018-08       Impact factor: 4.074

Review 2.  Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections.

Authors:  Ruben A V Dolmans; C H Edwin Boel; Miangela M Lacle; Johannes G Kusters
Journal:  Clin Microbiol Rev       Date:  2017-04       Impact factor: 26.132

Review 3.  [PCR-based detection of pathogens in clinical rheumatology].

Authors:  B Ehrenstein; U Reischl
Journal:  Z Rheumatol       Date:  2016-05       Impact factor: 1.372

4.  Gastrointestinal diagnosis of classical Whipple disease: clinical, endoscopic, and histopathologic features in 191 patients.

Authors:  Ute Günther; Verena Moos; Gabriel Offenmüller; Gerrit Oelkers; Walther Heise; Annette Moter; Christoph Loddenkemper; Thomas Schneider
Journal:  Medicine (Baltimore)       Date:  2015-04       Impact factor: 1.889

Review 5.  Whipple's Disease.

Authors:  Wilfried Obst; Ulrike von Arnim; Peter Malfertheiner
Journal:  Viszeralmedizin       Date:  2014-06

6.  Diagnostic Approach for Classic Compared With Localized Whipple Disease.

Authors:  Nicholas R Crews; Kelly A Cawcutt; Bobbi S Pritt; Robin Patel; Abinash Virk
Journal:  Open Forum Infect Dis       Date:  2018-06-13       Impact factor: 3.835

7.  Potential Role for Urine Polymerase Chain Reaction in the Diagnosis of Whipple's Disease.

Authors:  Annette Moter; Matthias Janneck; Manuel Wolters; Christof Iking-Konert; Alexandra Wiessner; Christoph Loddenkemper; Björn Hartleben; Marc Lütgehetmann; Julia Schmidt; Ulrike Langbehn; Sabrina Janssen; Anika Geelhaar-Karsch; Thomas Schneider; Verena Moos; Holger Rohde; Judith Kikhney; Thorsten Wiech
Journal:  Clin Infect Dis       Date:  2019-03-19       Impact factor: 9.079
  7 in total

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