Literature DB >> 24740078

Inhibition controls for qualitative real-time PCR assays: are they necessary for all specimen matrices?

S P Buckwalter1, L M Sloan1, S A Cunningham1, M J Espy1, J R Uhl1, M F Jones1, E A Vetter1, J Mandrekar1, F R Cockerill1, B S Pritt1, R Patel1, N L Wengenack2.   

Abstract

A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤ 1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Year:  2014        PMID： 24740078      PMCID： PMC4042775          DOI： 10.1128/JCM.03389-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

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