Literature DB >> 1599425

Kinetic parameters for the generation of endothelins-1,-2 and -3 by human cathepsin E.

P S Robinson1, W E Lees, J Kay, N D Cook.   

Abstract

The specific conversion of human endothelin (ET) precursors big ET-1, big ET-2 and big ET-3 into their respective ET by cathepsin E was examined. Comparable pH optima were obtained for ET-1 and ET-2 generation, whereas effective conversion of big ET-3 into ET-3 necessitated a lower pH value. Determination of kinetic parameters (Km, kcat.) for all three conversions indicated that the precursors were efficiently bound by cathepsin E. The significance of the values obtained for the catalytic-centre activities and the effect of a specific inhibitor are discussed.

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Year:  1992        PMID： 1599425      PMCID： PMC1132653          DOI： 10.1042/bj2840407

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Generation of human endothelin by cathepsin E.

Authors:  W E Lees; S Kalinka; J Meech; S J Capper; N D Cook; J Kay
Journal:  FEBS Lett       Date:  1990-10-29       Impact factor: 4.124

2.  Rapid release of endothelin and ATP from isolated aortic endothelial cells exposed to increased flow.

Authors:  P Milner; P Bodin; A Loesch; G Burnstock
Journal:  Biochem Biophys Res Commun       Date:  1990-07-31       Impact factor: 3.575

3.  Conversion of porcine big endothelin to endothelin by an extract from the porcine aortic endothelial cells.

Authors:  Y Matsumura; R Ikegawa; M Takaoka; S Morimoto
Journal:  Biochem Biophys Res Commun       Date:  1990-02-28       Impact factor: 3.575

4.  Stabilisation of cathepsin E by ATP.

Authors:  D J Thomas; A D Richards; R A Jupp; E Ueno; K Yamamoto; I M Samloff; B M Dunn; J Kay
Journal:  FEBS Lett       Date:  1989-01-30       Impact factor: 4.124

5.  Evidence for pepstatin-sensitive conversion of porcine big endothelin-1 to endothelin-1 by the endothelial cell extract.

Authors:  R Ikegawa; Y Matsumura; M Takaoka; S Morimoto
Journal:  Biochem Biophys Res Commun       Date:  1990-03-16       Impact factor: 3.575

6.  The pH dependence of the hydrolysis of chromogenic substrates of the type, Lys-Pro-Xaa-Yaa-Phe-(NO2)Phe-Arg-Leu, by selected aspartic proteinases: evidence for specific interactions in subsites S3 and S2.

Authors:  B M Dunn; M J Valler; C E Rolph; S I Foundling; M Jimenez; J Kay
Journal:  Biochim Biophys Acta       Date:  1987-06-17

7.  A systematic series of synthetic chromophoric substrates for aspartic proteinases.

Authors:  B M Dunn; M Jimenez; B F Parten; M J Valler; C E Rolph; J Kay
Journal:  Biochem J       Date:  1986-08-01       Impact factor: 3.857

Review 8.  Endothelins.

Authors:  G M Rubanyi; L H Botelho
Journal:  FASEB J       Date:  1991-09       Impact factor: 5.191

Review 9.  Molecular biology and biochemistry of the endothelins.

Authors:  M Yanagisawa; T Masaki
Journal:  Trends Pharmacol Sci       Date:  1989-09       Impact factor: 14.819

10.  The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes.

Authors:  A Inoue; M Yanagisawa; S Kimura; Y Kasuya; T Miyauchi; K Goto; T Masaki
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205
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