| Literature DB >> 1599260 |
Y Tan1, K J Miller.
Abstract
In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated from refrigerated raw milk. Sequence analysis revealed one major open reading frame of 1,428 nucleotides that was predicted to encode a protein with a molecular weight of 50,241. The predicted enzyme was found to contain an amino acid sequence highly homologous to the putative substrate-binding domain present within all lipases examined to date.Mesh:
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Year: 1992 PMID: 1599260 PMCID: PMC195611 DOI: 10.1128/aem.58.4.1402-1407.1992
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792