Literature DB >> 1599256

Conditions for protoplasting, regenerating, and transforming the calicheamicin producer, Micromonospora echinospora.

S F Love1, W M Maiese, D M Rothstein.   

Abstract

We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These protocols may be applied to other actinomycetes that have been difficult to transform. These methods also may facilitate the cloning of calicheamicin biosynthetic genes by genetic complementation.

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Year:  1992        PMID： 1599256      PMCID： PMC195603          DOI： 10.1128/aem.58.4.1376-1378.1992

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

Review 1.  Molecular genetics of polyketides and its comparison to fatty acid biosynthesis.

Authors:  D A Hopwood; D H Sherman
Journal:  Annu Rev Genet       Date:  1990       Impact factor: 16.830

2.  Isolation of mutants blocked in calicheamicin biosynthesis.

Authors:  D M Rothstein; S F Love
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490

Review 3.  Streptomyces cloning: useful recombinant DNA systems and a summation of cloned genes.

Authors:  P K Tomich
Journal:  Antimicrob Agents Chemother       Date:  1988-10       Impact factor: 5.191

4.  Temporally regulated tandem promoters in Micromonospora echinospora.

Authors:  E Z Baum; S F Love; D M Rothstein
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

5.  Efficient transformation of Micromonospora purpurea with pIJ702 plasmid.

Authors:  G H Kelemen; I Financsek; M Járai
Journal:  J Antibiot (Tokyo)       Date:  1989-02       Impact factor: 2.649

6.  An improved method for the preparation of Streptomycetes and Micromonospora protoplasts.

Authors:  H Ogawa; S Imai; A Satoh; M Kojima
Journal:  J Antibiot (Tokyo)       Date:  1983-02       Impact factor: 2.649

7.  Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator.

Authors:  J M Ward; G R Janssen; T Kieser; M J Bibb; M J Buttner; M J Bibb
Journal:  Mol Gen Genet       Date:  1986-06

8.  Calicheamicin gamma 1I: an antitumor antibiotic that cleaves double-stranded DNA site specifically.

Authors:  N Zein; A M Sinha; W J McGahren; G A Ellestad
Journal:  Science       Date:  1988-05-27       Impact factor: 47.728

9.  Calicheamicins, a novel family of antitumor antibiotics: taxonomy, fermentation and biological properties.

Authors:  W M Maiese; M P Lechevalier; H A Lechevalier; J Korshalla; N Kuck; A Fantini; M J Wildey; J Thomas; M Greenstein
Journal:  J Antibiot (Tokyo)       Date:  1989-04       Impact factor: 2.649

10.  Calicheamicins, a novel family of antitumor antibiotics. 3. Isolation, purification and characterization of calicheamicins beta 1Br, gamma 1Br, alpha 2I, alpha 3I, beta 1I, gamma 1I and delta 1I.

Authors:  M D Lee; J K Manning; D R Williams; N A Kuck; R T Testa; D B Borders
Journal:  J Antibiot (Tokyo)       Date:  1989-07       Impact factor: 2.649
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  3 in total

1.  Mutations in the P1 promoter region of Micromonospora echinospora.

Authors:  L S Lin; D M Rothstein
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

2.  Vector systems allowing efficient autonomous or integrative gene cloning in Micromonospora sp. strain 40027.

Authors:  Xiaohua Li; Xiufen Zhou; Zixin Deng
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

3.  Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set.

Authors:  Je Won Park; Jay Sung Joong Hong; Niranjan Parajuli; Won Seok Jung; Sung Ryeol Park; Si-Kyu Lim; Jae Kyung Sohng; Yeo Joon Yoon
Journal:  Proc Natl Acad Sci U S A       Date:  2008-06-12       Impact factor: 11.205
  3 in total

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