Literature DB >> 15986149

Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry. Part I--Immunometric assays.

Niko J Meltola1, Mika J Kettunen, Aleksi E Soini.   

Abstract

Seven different two-photon excitable dipyrrylmetheneboron difluoride labels (dipyrrylmethene-BF(2) labels) and a frequently used TAMRA label were conjugated to mouse IgG against alpha-fetoprotein in variable substitution degrees. Altogether 40 IgG conjugates were prepared, and studied with respect to one-photon absorption and emission properties, and two-photon fluorescence efficiency using 1064 nm laser as illumination source. Performance of the IgG conjugates as tracers in a separation-free immunometric assay of alpha-fetoprotein was evaluated using two-photon excitation assay technology, ArcDia TPX. The results show that the dipyrrylmethene-BF(2) labels provide subpicomolar sensitivity, which is an order of magnitude better than that of TAMRA label. The effect of chromophore structure and substitution degree of IgG-label conjugates on the assay performance is discussed.

Entities:  

Year:  2005        PMID： 15986149     DOI： 10.1007/s10895-005-2622-3

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  12 in total

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Journal:  Anal Chem       Date:  2002-11-15       Impact factor: 6.986

2.  Reaction kinetics of a two-photon excitation microparticle based immunoassay--from modelling to practice.

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Journal:  Biophys Chem       Date:  2003-08-01       Impact factor: 2.352

3.  Electrochemiluminescence detection for development of immunoassays and DNA probe assays for clinical diagnostics.

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Journal:  Clin Chem       Date:  1991-09       Impact factor: 8.327

4.  Hydrophilic labeling reagents of dipyrrylmethene-BF2 dyes for two-photon excited fluorometry: syntheses and photophysical characterization.

Authors:  Niko J Meltola; Rina Wahlroos; Aleksi E Soini
Journal:  J Fluoresc       Date:  2004-09       Impact factor: 2.217

5.  Single-molecule detection by two-photon-excited fluorescence.

Authors:  J Mertz; C Xu; W W Webb
Journal:  Opt Lett       Date:  1995-12-15       Impact factor: 3.776

6.  Fluorescent tetramethyl rhodamine derivatives of alpha-bungarotoxin: preparation, separation, and characterization.

Authors:  P Ravdin; D Axelrod
Journal:  Anal Biochem       Date:  1977-06       Impact factor: 3.365

7.  A new microvolume technique for bioaffinity assays using two-photon excitation.

Authors:  P Hänninen; A Soini; N Meltola; J Soini; J Soukka; E Soini
Journal:  Nat Biotechnol       Date:  2000-05       Impact factor: 54.908

8.  Advanced multiplexed analysis with the FlowMetrix system.

Authors:  R J Fulton; R L McDade; P L Smith; L J Kienker; J R Kettman
Journal:  Clin Chem       Date:  1997-09       Impact factor: 8.327

9.  Two-photon excitation fluorometric measurement of homogeneous microparticle immunoassay for C-reactive protein.

Authors:  Matti E Waris; Niko J Meltola; Juhani T Soini; Erkki Soini; Olli J Peltola; Pekka E Hänninen
Journal:  Anal Biochem       Date:  2002-10-01       Impact factor: 3.365

10.  Fluorescent nanoparticles as labels for immunometric assay of C-reactive protein using two-photon excitation assay technology.

Authors:  Janne O Koskinen; Jonne Vaarno; Niko J Meltola; Juhani T Soini; Pekka E Hänninen; Juhani Luotola; Matti E Waris; Aleksi E Soini
Journal:  Anal Biochem       Date:  2004-05-15       Impact factor: 3.365
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  5 in total

1.  Effect of polystyrene microsphere surface to fluorescence lifetime under two-photon excitation.

Authors:  Marko E Tirri; Rina Wahlroos; Niko J Meltola; Juha Toivonen; Pekka E Hänninen
Journal:  J Fluoresc       Date:  2006-09-16       Impact factor: 2.217

2.  Evaluation of the TPX MRSA assay for the detection of methicillin-resistant Staphylococcus aureus.

Authors:  T Stenholm; A J Hakanen; S Salmenlinna; S Pihlasalo; H Härmä; P E Hänninen; P Huovinen; J Vuopio; P Kotilainen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-04-02       Impact factor: 3.267

3.  Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry. Part II--Nucleic acid hybridization assays.

Authors:  Niko J Meltola; Jonne Vaarno; Aleksi E Soini
Journal:  J Fluoresc       Date:  2005-05       Impact factor: 2.217

4.  Rapid method for detection of influenza a and B virus antigens by use of a two-photon excitation assay technique and dry-chemistry reagents.

Authors:  Janne O Koskinen; Raija Vainionpää; Niko J Meltola; Jori Soukka; Pekka E Hänninen; Aleksi E Soini
Journal:  J Clin Microbiol       Date:  2007-09-12       Impact factor: 5.948

5.  Methicillin-resistant Staphylococcus aureus screening by online immunometric monitoring of bacterial growth under selective pressure.

Authors:  Teppo Stenholm; Antti J Hakanen; Jonne Vaarno; Sari Pihlasalo; Perttu Terho; Pekka E Hänninen; Jaana Vuopio-Varkila; Pentti Huovinen; Pirkko Kotilainen
Journal:  Antimicrob Agents Chemother       Date:  2009-09-14       Impact factor: 5.191
  5 in total

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