Literature DB >> 15986150

Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry. Part II--Nucleic acid hybridization assays.

Niko J Meltola1, Jonne Vaarno, Aleksi E Soini.   

Abstract

Five two-photon excitable dipyrrylmetheneboron difluoride labels (dipyrrylmethene-BF(2) labels) with fluorescence emission maximum between 530 and 590 nm, and a frequently used rhodamine label, TAMRA, were conjugated to aminomodified oligonucleotides. The performance of the labeled oligonucleotides was studied in a separation-free nucleic acid hybridization assay using ArcDia TPX bioaffinity assay technology. The results show that oligonucleotide conjugates of dipyrrylmethene-BF(2) labels provide higher two-photon excited fluorescence yield and better assay sensitivity than corresponding TAMRA conjugate. The effect of conjugation on photophysical properties of the labels and performance of the labeled oligonucleotides in separation-free hybridization assay is discussed.

Entities:  

Year:  2005        PMID： 15986150     DOI： 10.1007/s10895-005-2623-2

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  32 in total

Review 1.  The application of DNA microarrays in gene expression analysis.

Authors:  N L van Hal; O Vorst; A M van Houwelingen; E J Kok; A Peijnenburg; A Aharoni; A J van Tunen; J Keijer
Journal:  J Biotechnol       Date:  2000-03-31       Impact factor: 3.307

2.  Detection of PCR products using self-probing amplicons and fluorescence.

Authors:  D Whitcombe; J Theaker; S P Guy; T Brown; S Little
Journal:  Nat Biotechnol       Date:  1999-08       Impact factor: 54.908

Review 3.  The identification of microorganisms by fluorescence in situ hybridisation.

Authors:  R Amann; B M Fuchs; S Behrens
Journal:  Curr Opin Biotechnol       Date:  2001-06       Impact factor: 9.740

Review 4.  Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes.

Authors:  Michael Wagner; Matthias Horn; Holger Daims
Journal:  Curr Opin Microbiol       Date:  2003-06       Impact factor: 7.934

5.  Hydrophilic labeling reagents of dipyrrylmethene-BF2 dyes for two-photon excited fluorometry: syntheses and photophysical characterization.

Authors:  Niko J Meltola; Rina Wahlroos; Aleksi E Soini
Journal:  J Fluoresc       Date:  2004-09       Impact factor: 2.217

6.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

7.  Two-photon laser scanning fluorescence microscopy.

Authors:  W Denk; J H Strickler; W W Webb
Journal:  Science       Date:  1990-04-06       Impact factor: 47.728

8.  A primer-guided nucleotide incorporation assay in the genotyping of apolipoprotein E.

Authors:  A C Syvänen; K Aalto-Setälä; L Harju; K Kontula; H Söderlund
Journal:  Genomics       Date:  1990-12       Impact factor: 5.736

9.  Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay.

Authors:  H Hakala; P Virta; H Salo; H Lönnberg
Journal:  Nucleic Acids Res       Date:  1998-12-15       Impact factor: 16.971

10.  Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Authors:  K J Livak; S J Flood; J Marmaro; W Giusti; K Deetz
Journal:  PCR Methods Appl       Date:  1995-06
View more
  2 in total

1.  Two-photon excited fluorescence energy transfer: a study based on oligonucleotide rulers.

Authors:  Rina Wahlroos; Juha Toivonen; Marko Tirri; Pekka Hänninen
Journal:  J Fluoresc       Date:  2006-05-16       Impact factor: 2.217

2.  Rapid method for detection of influenza a and B virus antigens by use of a two-photon excitation assay technique and dry-chemistry reagents.

Authors:  Janne O Koskinen; Raija Vainionpää; Niko J Meltola; Jori Soukka; Pekka E Hänninen; Aleksi E Soini
Journal:  J Clin Microbiol       Date:  2007-09-12       Impact factor: 5.948
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.