Literature DB >> 1598204

Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome.

P López-García1, J P Abad, C Smith, R Amils.   

Abstract

Pulsed field gel electrophoresis (PFG) has been used to study the genomic organization of the halophilic archaeon Haloferax mediterranei. Analysis of the different genomic elements as well as the restriction patterns obtained with several endonucleases revealed that this microorganism has a circular chromosome of 2.9 Mb and, at least, three extrachromosomal elements of 490, 320 and 130 kb respectively. The complete physical map of the chromosome for the endonucleases PacI and BamHI has been constructed, and several BcII, BgIII and DraI restriction fragments have been aligned on these maps. The localization of heterologous and homologous genes on the physical map, including those for rRNA, lay the ground work for the construction of a genetic map.

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Year:  1992        PMID: 1598204      PMCID: PMC312379          DOI: 10.1093/nar/20.10.2459

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  19 in total

1.  Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2.

Authors:  R L Charlebois; L C Schalkwyk; J D Hofman; W F Doolittle
Journal:  J Mol Biol       Date:  1991-12-05       Impact factor: 5.469

2.  Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis.

Authors:  J J Lee; H O Smith
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

3.  Strategies for mapping and cloning macroregions of mammalian genomes.

Authors:  C L Smith; S K Lawrance; G A Gillespie; C R Cantor; S M Weissman; F S Collins
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Purification, specific fragmentation, and separation of large DNA molecules.

Authors:  C L Smith; C R Cantor
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  A physical map of the Escherichia coli K12 genome.

Authors:  C L Smith; J G Econome; A Schutt; S Klco; C R Cantor
Journal:  Science       Date:  1987-06-12       Impact factor: 47.728

6.  ISH51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, Halobacterium volcanii.

Authors:  J D Hofman; L C Schalkwyk; W F Doolittle
Journal:  Nucleic Acids Res       Date:  1986-09-11       Impact factor: 16.971

7.  Genome mapping in halobacteria.

Authors:  R L Charlebois; J D Hofman; L C Schalkwyk; W L Lam; W F Doolittle
Journal:  Can J Microbiol       Date:  1989-01       Impact factor: 2.419

8.  In vitro reassembly of active large ribosomal subunits of the halophilic archaebacterium Haloferax mediterranei.

Authors:  M E Sanchez; D Ureña; R Amils; P Londei
Journal:  Biochemistry       Date:  1990-10-02       Impact factor: 3.162

9.  Mechanisms of genetic variability in Halobacterium halobium: the purple membrane and gas vesicle mutations.

Authors:  S DasSarma
Journal:  Can J Microbiol       Date:  1989-01       Impact factor: 2.419

10.  Stimulation of an alpha like DNA polymerase by v-myc related protein of Halobacterium halobium.

Authors:  K Ben-Mahrez; W Sougakoff; M Nakayama; M Kohiyama
Journal:  Arch Microbiol       Date:  1988-01       Impact factor: 2.552

View more
  9 in total

1.  A physical map of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius 7 chromosome.

Authors:  S Kondo; A Yamagishi; T Oshima
Journal:  J Bacteriol       Date:  1993-03       Impact factor: 3.490

2.  Comparative genomic analysis of the Haloferax volcanii DS2 and Halobacterium salinarium GRB contig maps reveals extensive rearrangement.

Authors:  A St Jean; R L Charlebois
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

3.  Physical map and set of overlapping cosmid clones representing the genome of the archaeon Halobacterium sp. GRB.

Authors:  A St Jean; B A Trieselmann; R L Charlebois
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

4.  Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity.

Authors:  C Ferrer; F J Mojica; G Juez; F Rodríguez-Valera
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490

5.  Isolation, sequence, and expression of the gene encoding halocin H4, a bacteriocin from the halophilic archaeon Haloferax mediterranei R4.

Authors:  J Cheung; K J Danna; E M O'Connor; L B Price; R F Shand
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

6.  Complete genome sequence of the metabolically versatile halophilic archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) producer.

Authors:  Jing Han; Fan Zhang; Jing Hou; Xiaoqing Liu; Ming Li; Hailong Liu; Lei Cai; Bing Zhang; Yaping Chen; Jian Zhou; Songnian Hu; Hua Xiang
Journal:  J Bacteriol       Date:  2012-08       Impact factor: 3.490

7.  Comparison of four phaC genes from Haloferax mediterranei and their function in different PHBV copolymer biosyntheses in Haloarcula hispanica.

Authors:  Jing Han; Ming Li; Jing Hou; Linping Wu; Jian Zhou; Hua Xiang
Journal:  Saline Syst       Date:  2010-08-20

8.  Physical map of the Methanobacterium thermoautotrophicum Marburg chromosome.

Authors:  R Stettler; T Leisinger
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

9.  Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei.

Authors:  P López-García; A St Jean; R Amils; R L Charlebois
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490

  9 in total

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