Literature DB >> 2842319

Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis.

J J Lee1, H O Smith.   

Abstract

The four restriction enzymes ApaI (5'-GGGCCC), EagI (5'-CGGCCG), NaeI (5'-GCCGGC), and SmaI (5'-CCCGGG) were found to produce distributions of DNA fragment sizes useful for mapping of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. ApaI produced 21 fragments (range, 1.6 to 305 kilobases [kb]), EagI yielded 30 fragments (0.6 to 339 kb), NaeI produced 32 fragments (2.3 to 290 kb), and SmaI yielded 16 fragments (6.0 to 377 kb). Summation of the fragment lengths in each digest yielded estimates for the size of the H. influenzae chromosome ranging from 1,834 kb.

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Year:  1988        PMID： 2842319      PMCID： PMC211461          DOI： 10.1128/jb.170.9.4402-4405.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

1.  ELECTRON MICROSCOPY OF DNA FROM HEMOPHILUS INFLUENZAE.

Authors:  L A MACHATTIE; K I BERNE; C A THOMAS
Journal:  J Mol Biol       Date:  1965-03       Impact factor: 5.469

2.  Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5'-triphosphate-dependent deoxyribonuclease activity.

Authors:  K W Wilcox; H O Smith
Journal:  J Bacteriol       Date:  1975-05       Impact factor: 3.490

3.  A physical map of the Escherichia coli K12 genome.

Authors:  C L Smith; J G Econome; A Schutt; S Klco; C R Cantor
Journal:  Science       Date:  1987-06-12       Impact factor: 47.728

4.  DNA methylases of Hemophilus influenzae Rd. I. Purification and properties.

Authors:  P H Roy; H O Smith
Journal:  J Mol Biol       Date:  1973-12-25       Impact factor: 5.469

5.  Defined nongrowth media for stage II development of competence in Haemophilus influenzae.

Authors:  R M Herriott; E M Meyer; M Vogt
Journal:  J Bacteriol       Date:  1970-02       Impact factor: 3.490

6.  Genetic and physical map of the chromosome of Hemophilus influenzae.

Authors:  J Michalka; S H Goodgal
Journal:  J Mol Biol       Date:  1969-10-28       Impact factor: 5.469

7.  Separation of large DNA molecules by contour-clamped homogeneous electric fields.

Authors:  G Chu; D Vollrath; R W Davis
Journal:  Science       Date:  1986-12-19       Impact factor: 47.728

8.  Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation.

Authors:  K C Zoon; J J Scocca
Journal:  J Bacteriol       Date:  1975-08       Impact factor: 3.490

9.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Transfer of genetic information within a colony of Haemophilus influenzae.

Authors:  J H Stuy
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490
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  26 in total

1.  rec-2-dependent phage recombination in Haemophilus influenzae.

Authors:  D M Kupfer; D McCarthy
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

2.  Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.

Authors:  D C Krause; C B Mawn
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

3.  Determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in Yersinia pestis by pulsed-field gel electrophoresis.

Authors:  T S Lucier; R R Brubaker
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

4.  Genomic organization of the halophilic archaeon Haloferax mediterranei: physical map of the chromosome.

Authors:  P López-García; J P Abad; C Smith; R Amils
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

Review 5.  New approaches for physical mapping of small genomes.

Authors:  C L Smith; G Condemine
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

6.  Genome analysis of Bradyrhizobium japonicum serocluster 123 field isolates by using field inversion gel electrophoresis.

Authors:  B W Sobral; M J Sadowsky; A G Atherly
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

7.  Determination of genome size of Pseudomonas aeruginosa by PFGE: analysis of restriction fragments.

Authors:  J S Hector; A R Johnson
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

8.  A peroxide/ascorbate-inducible catalase from Haemophilus influenzae is homologous to the Escherichia coli katE gene product.

Authors:  W R Bishai; H O Smith; G J Barcak
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

9.  The genomes of the family Rhizobiaceae: size, stability, and rarely cutting restriction endonucleases.

Authors:  B W Sobral; R J Honeycutt; A G Atherly
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

10.  Dynamics of long-term colonization of respiratory tract by Haemophilus influenzae in cystic fibrosis patients shows a marked increase in hypermutable strains.

Authors:  Federico Román; Rafael Cantón; María Pérez-Vázquez; Fernando Baquero; José Campos
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948
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