Literature DB >> 15956443

Extraction of Mycobacterium tuberculosis DNA: a question of containment.

Wendy Somerville1, Louise Thibert, Kevin Schwartzman, Marcel A Behr.   

Abstract

DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.

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Year:  2005        PMID： 15956443      PMCID： PMC1151963          DOI： 10.1128/JCM.43.6.2996-2997.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  The efficacy of the heat killing of Mycobacterium tuberculosis.

Authors:  C Doig; A L Seagar; B Watt; K J Forbes
Journal:  J Clin Pathol       Date:  2002-10       Impact factor: 3.411

2.  Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis.

Authors:  D van Soolingen; P W Hermans; P E de Haas; D R Soll; J D van Embden
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

3.  Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.

Authors:  J D van Embden; M D Cave; J T Crawford; J W Dale; K D Eisenach; B Gicquel; P Hermans; C Martin; R McAdam; T M Shinnick
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

4.  Inactivation of Mycobacterium tuberculosis for DNA typing analysis.

Authors:  P Bemer-Melchior; H B Drugeon
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

5.  Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.

Authors:  P M Small; N B McClenny; S P Singh; G K Schoolnik; L S Tompkins; P A Mickelsen
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

6.  Comparison of the Septi-Chek AFB and BACTEC systems and conventional culture for recovery of mycobacteria.

Authors:  D L Sewell; A L Rashad; W J Rourke; S L Poor; J A McCarthy; M A Pfaller
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

7.  Rendering of mycobacteria safe for molecular diagnostic studies and development of a lysis method for strand displacement amplification and PCR.

Authors:  P Zwadyk; J A Down; N Myers; M S Dey
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948
  7 in total
  67 in total

1.  Inactivation of cultured Mycobacterium tuberculosis organisms prior to DNA extraction.

Authors:  Zoheira Djelouagji; Michel Drancourt
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

2.  Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.

Authors:  Robin Warren; Marianna de Kock; Erica Engelke; Roseline Myburgh; Nico Gey van Pittius; Thomas Victor; Paul van Helden
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

3.  Mutation detection and accurate diagnosis of extensively drug-resistant tuberculosis: report from a tertiary care center in India.

Authors:  Kanchan Ajbani; Camilla Rodrigues; Shubhada Shenai; Ajita Mehta
Journal:  J Clin Microbiol       Date:  2011-02-02       Impact factor: 5.948

4.  Use of sloppy molecular beacon probes for identification of mycobacterial species.

Authors:  Hiyam H El-Hajj; Salvatore A E Marras; Sanjay Tyagi; Elena Shashkina; Mini Kamboj; Timothy E Kiehn; Michael S Glickman; Fred Russell Kramer; David Alland
Journal:  J Clin Microbiol       Date:  2009-01-26       Impact factor: 5.948

5.  Mutations within embCAB Are Associated with Variable Level of Ethambutol Resistance in Mycobacterium tuberculosis Isolates from China.

Authors:  Qing Sun; Tong-Yang Xiao; Hai-Can Liu; Xiu-Qin Zhao; Zhi-Guang Liu; Ya-Nan Li; Hao Zeng; Li-Li Zhao; Kang-Lin Wan
Journal:  Antimicrob Agents Chemother       Date:  2017-12-21       Impact factor: 5.191

6.  Rapid diagnosis of extensively drug-resistant tuberculosis by use of a reverse line blot hybridization assay.

Authors:  Kanchan Ajbani; Anjali Shetty; Ajita Mehta; Camilla Rodrigues
Journal:  J Clin Microbiol       Date:  2011-05-25       Impact factor: 5.948

7.  Prevalence of mutations conferring resistance among multi- and extensively drug-resistant Mycobacterium tuberculosis isolates in China.

Authors:  Yan Chen; Bing Zhao; Hai-can Liu; Qing Sun; Xiu-qin Zhao; Zhi-guang Liu; Kang-lin Wan; Li-li Zhao
Journal:  J Antibiot (Tokyo)       Date:  2015-10-21       Impact factor: 2.649

8.  Genomic Analysis of the Evolution of Fluoroquinolone Resistance in Mycobacterium tuberculosis Prior to Tuberculosis Diagnosis.

Authors:  Danfeng Zhang; James E Gomez; Jung-Yien Chien; Nathan Haseley; Christopher A Desjardins; Ashlee M Earl; Po-Ren Hsueh; Deborah T Hung
Journal:  Antimicrob Agents Chemother       Date:  2016-10-21       Impact factor: 5.191

9.  The DosR dormancy regulator of Mycobacterium tuberculosis stimulates the Na(+)/K (+) and Ca (2+) ATPase activities in plasma membrane vesicles of mycobacteria.

Authors:  Paola A Pulido; Lorena Novoa-Aponte; Nicolás Villamil; Carlos Y Soto
Journal:  Curr Microbiol       Date:  2014-06-18       Impact factor: 2.188

10.  Major Mycobacterium tuberculosis lineages associate with patient country of origin.

Authors:  Michael B Reed; Victoria K Pichler; Fiona McIntosh; Alicia Mattia; Ashley Fallow; Speranza Masala; Pilar Domenech; Alice Zwerling; Louise Thibert; Dick Menzies; Kevin Schwartzman; Marcel A Behr
Journal:  J Clin Microbiol       Date:  2009-02-11       Impact factor: 5.948
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