Xue-Min Yang1, Jian-Ping Liang1, Xiao-Juan Huang1, Xiang-Rong Wang1, Yang Sun2, Chen Dong1, Ya-Li Cui3,4, Wen-Li Hui5,6. 1. The College of Life Science, Northwest University, Xi'an, 710069, Shanxi, China. 2. Data Center of Shaanxi Provincial People's Hospital, Xi'an, 710069, Shanxi, China. 3. The College of Life Science, Northwest University, Xi'an, 710069, Shanxi, China. yalicui@nwu.edu.cn. 4. Shanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, 710077, Shanxi, China. yalicui@nwu.edu.cn. 5. The College of Life Science, Northwest University, Xi'an, 710069, Shanxi, China. huiwl@nwu.edu.cn. 6. Shanxi Provincial Engineering Research Center for Nano-Biomedical Detection, Xi'an, 710077, Shanxi, China. huiwl@nwu.edu.cn.
Abstract
BACKGROUND: Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it's involvement in various pathophysiological conditions. RESULTS: In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 min. The detection limit was 0.75 ng and results could be obtained in 5 min using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples. CONCLUSIONS: After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.
BACKGROUND:Angiotensin-converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. The wide distribution and multifunctional properties of ACE suggest it's involvement in various pathophysiological conditions. RESULTS: In this study, a novel visual detection method for ACE I/D polymorphisms was designed by integrating direct PCR without the need for DNA extraction using gold magnetic nanoparticles (GMNPs)-based lateral flow assay (LFA) biosensor. The entire detection procedure could enable the genotyping of clinical samples in about 80 min. The detection limit was 0.75 ng and results could be obtained in 5 min using the LFA device. Three hundred peripheral blood samples were analyzed using the direct PCR-LFA system and then verified by sequencing to determine accuracy and repeatability. A clinical preliminary study was then performed to analyze a total of 633 clinical samples. CONCLUSIONS: After grouping based on age, we found a significant difference between the genotypes and the age of patients in the CHD group. The introduction of this method into clinical practice may be helpful for the diagnosis of diseases caused by large fragment gene insertions/deletions.
Authors: G A Stavroulakis; T K Makris; P G Krespi; A N Hatzizacharias; A E Gialeraki; G Anastasiadis; P Triposkiadis; M Kyriakidis Journal: Cardiovasc Drugs Ther Date: 2000-08 Impact factor: 3.727
Authors: Denise S Pinheiro; Rodrigo S Santos; Paulo C B Veiga Jardim; Elisangela G Silva; Angela A S Reis; Gustavo R Pedrino; Cirano J Ulhoa Journal: PLoS One Date: 2019-08-20 Impact factor: 3.240