Literature DB >> 15900567

Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G.

Vardan K Gasparyan1.   

Abstract

Conjugation of immunoglobulin G (IgG) from rabbit serum with colloidal gold for sol agglutination assay was conducted. Effects of various buffer systems on conjugation and stability of sols were studied. Colloidal gold assay for detection of human myoglobin and C-reactive protein was developed. It was shown that rabbit IgG had a poor stabilizing effect on colloidal gold sol and therefore that it cannot be effectively used in such assays. In contrast, hen egg immunoglobulin Y (IgY) had excellent stabilizing properties used in various buffer systems and in a wide range of concentrations. IgY was considered as a useful alternative for rabbit antibodies in gold sol agglutination assay. Copyright 2005 Wiley-Liss, Inc.

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Year:  2005        PMID： 15900567      PMCID： PMC6807854          DOI： 10.1002/jcla.20065

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN： 0887-8013            Impact factor:   2.352


  12 in total

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Authors:  G W Hammond; G S Ahluwalia; P R Hazelton
Journal:  J Infect Dis       Date:  1984-06       Impact factor: 5.226

5.  Latex agglutination test for rapid detection of bacterial antigens in body fluids.

Authors:  A Kumar; B L Congeni; G A Nankervis
Journal:  Ann Clin Lab Sci       Date:  1980 Sep-Oct       Impact factor: 1.256

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Authors:  V K Gasparyan
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7.  Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG.

Authors:  J C Jensenius; I Andersen; J Hau; M Crone; C Koch
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

8.  Purification of C-reactive protein on DEAE-cellulose by a simple two-step procedure utilizing the calcium-dependency of the protein.

Authors:  U Johnson; K Prellner
Journal:  Biochim Biophys Acta       Date:  1977-12-20

9.  General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha.

Authors:  G Camenisch; M Tini; D Chilov; I Kvietikova; V Srinivas; J Caro; P Spielmann; R H Wenger; M Gassmann
Journal:  FASEB J       Date:  1999-01       Impact factor: 5.191

10.  A sol particle agglutination assay for human chorionic gonadotrophin.

Authors:  J H Leuvering; P J Thal; M Van der Waart; A H Schuurs
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303
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  1 in total

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