Literature DB >> 588590

Purification of C-reactive protein on DEAE-cellulose by a simple two-step procedure utilizing the calcium-dependency of the protein.

U Johnson, K Prellner.   

Abstract

A simple procedure for isolating C-reactive protein is presented. The method consists of two chromatographic separations on diethylaminoethyl (DEAE)-cellulose columns and utilized the difference between the binding of C-reactive protein to DEAE in the presence and absence of calcium. Electrophoretically pure C-reactive protein can be recovered from serum or ascitic fluid with a yield of approx. 50--60% within one day.

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Year:  1977        PMID: 588590     DOI: 10.1016/0005-2795(77)90390-7

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  3 in total

1.  Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G.

Authors:  Vardan K Gasparyan
Journal:  J Clin Lab Anal       Date:  2005       Impact factor: 2.352

2.  Synthesis of water-soluble CdSe quantum dots with various fluorescent properties and their application in immunoassay for determination of C-reactive protein.

Authors:  V K Gasparyan
Journal:  J Fluoresc       Date:  2014-07-18       Impact factor: 2.217

Review 3.  An overview on human serum lectins.

Authors:  S Beulaja Manikandan; R Manikandan; M Arumugam; P Mullainadhan
Journal:  Heliyon       Date:  2020-08-27
  3 in total

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