Literature DB >> 15893936

Techniques to measure lipase and esterase activity in vitro.

Dean Gilham1, Richard Lehner.   

Abstract

Lipases and esterases constitute a large category of enzymes. They are ubiquitous in nature, found in bacteria, fungi, and animals. The family members address a wide variety of structurally diverse substrates. Appropriately, a large number of assays have been developed to analyze their activity in vitro. Here, we present an overview of these enzymes, along with protocols for common assays performed in solution. An emphasis is placed on assays for enzymes that can hydrolyze triacylglycerols.

Entities:  

Mesh:

Substances:

Year:  2005        PMID: 15893936     DOI: 10.1016/j.ymeth.2004.11.003

Source DB:  PubMed          Journal:  Methods        ISSN: 1046-2023            Impact factor:   3.608


  29 in total

1.  Pro- and antiatherogenic effects of a dominant-negative P465L mutation of peroxisome proliferator-activated receptor-γ in apolipoprotein E-Null mice.

Authors:  Avani A Pendse; Lance A Johnson; Hyung-Suk Kim; Marcus McNair; C Taylor Nipp; Carolyn Wilhelm; Nobuyo Maeda
Journal:  Arterioscler Thromb Vasc Biol       Date:  2012-04-26       Impact factor: 8.311

Review 2.  Marine Bacterial Esterases: Emerging Biocatalysts for Industrial Applications.

Authors:  Noora Barzkar; Muhammad Sohail; Saeid Tamadoni Jahromi; Mohsen Gozari; Sajjad Poormozaffar; Reza Nahavandi; Mahmoud Hafezieh
Journal:  Appl Biochem Biotechnol       Date:  2021-01-07       Impact factor: 2.926

3.  Gene cloning and characterization of a novel highly organic solvent tolerant lipase from Proteus sp. SW1 and its application for biodiesel production.

Authors:  Wirongrong Whangsuk; Pareenart Sungkeeree; Sirinthra Thiengmag; Jarunee Kerdwong; Ratiboot Sallabhan; Skorn Mongkolsuk; Suvit Loprasert
Journal:  Mol Biotechnol       Date:  2013-01       Impact factor: 2.695

4.  DNA-polyfluorophore excimers as sensitive reporters for esterases and lipases.

Authors:  Nan Dai; Yin Nah Teo; Eric T Kool
Journal:  Chem Commun (Camb)       Date:  2010-01-22       Impact factor: 6.222

5.  Hepatic deficiency of low density lipoprotein receptor-related protein-1 reduces high density lipoprotein secretion and plasma levels in mice.

Authors:  Joshua E Basford; Lauren Wancata; Susanna M Hofmann; R A Gangani D Silva; W Sean Davidson; Philip N Howles; David Y Hui
Journal:  J Biol Chem       Date:  2011-02-22       Impact factor: 5.157

6.  LipC (Rv0220) is an immunogenic cell surface esterase of Mycobacterium tuberculosis.

Authors:  Guomiao Shen; Krishna Singh; Dinesh Chandra; Carole Serveau-Avesque; Damien Maurin; Stéphane Canaan; Rupak Singla; Digambar Behera; Suman Laal
Journal:  Infect Immun       Date:  2011-10-28       Impact factor: 3.441

7.  Biodegradable lipids enabling rapidly eliminated lipid nanoparticles for systemic delivery of RNAi therapeutics.

Authors:  Martin A Maier; Muthusamy Jayaraman; Shigeo Matsuda; Ju Liu; Scott Barros; William Querbes; Ying K Tam; Steven M Ansell; Varun Kumar; June Qin; Xuemei Zhang; Qianfan Wang; Sue Panesar; Renta Hutabarat; Mary Carioto; Julia Hettinger; Pachamuthu Kandasamy; David Butler; Kallanthottathil G Rajeev; Bo Pang; Klaus Charisse; Kevin Fitzgerald; Barbara L Mui; Xinyao Du; Pieter Cullis; Thomas D Madden; Michael J Hope; Muthiah Manoharan; Akin Akinc
Journal:  Mol Ther       Date:  2013-06-25       Impact factor: 11.454

8.  Mechanism for endogenously expressed ApoE modulation of adipocyte very low density lipoprotein metabolism: role in endocytic and lipase-mediated metabolic pathways.

Authors:  Zhi Hua Huang; Richard D Minshall; Theodore Mazzone
Journal:  J Biol Chem       Date:  2009-09-18       Impact factor: 5.157

9.  Inhibition of carboxylesterase 1 is associated with cholesteryl ester retention in human THP-1 monocyte/macrophages.

Authors:  J Allen Crow; Brandy L Middleton; Abdolsamad Borazjani; M Jason Hatfield; Philip M Potter; Matthew K Ross
Journal:  Biochim Biophys Acta       Date:  2008-08-05

10.  Arylacetamide deacetylase attenuates fatty-acid-induced triacylglycerol accumulation in rat hepatoma cells.

Authors:  Vivien Lo; Bruce Erickson; Michaela Thomason-Hughes; Kerry W S Ko; Vernon W Dolinsky; Randy Nelson; Richard Lehner
Journal:  J Lipid Res       Date:  2009-08-04       Impact factor: 5.922

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.