| Literature DB >> 22371263 |
Wirongrong Whangsuk1, Pareenart Sungkeeree, Sirinthra Thiengmag, Jarunee Kerdwong, Ratiboot Sallabhan, Skorn Mongkolsuk, Suvit Loprasert.
Abstract
Proteus sp. SW1 was found to produce an extracellular solvent tolerant lipase. The gene, lipA, encoding a bacterial lipase, was cloned from total Proteus sp. SW1 DNA. lipA was predicted to encode a 287 amino acid protein of 31.2 kDa belonging to the Group I proteobacterial lipases. Purified His-tagged LipA exhibited optimal activity at pH 10.0 and 55°C. It was highly stable in organic solvents retaining 112% of its activity in 100% isopropanol after 24 h, and exhibited more than 200% of its initial activity upon exposure to 60% acetone, ethanol, and hexane for 18 h. Biodiesel synthesis reactions, using a single step addition of 13% an acyl acceptor ethanol, showed that LipA was highly effective at converting palm oil into biodiesel.Entities:
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Year: 2013 PMID： 22371263 DOI： 10.1007/s12033-012-9518-7
Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695