| Literature DB >> 15868409 |
C S Lane1.
Abstract
Over the last 20 years, mass spectrometry-based proteomics has become an indispensable tool in the cellular and molecular life sciences. This has been enabled by the 'soft ionisation' techniques of electrospray and matrix-assisted laser desorption-ionisation, which allow the gentle ionisation and vaporisation of large, thermally labile biomolecules. Innovative instrumentation designs and biochemical strategies have brought success in the large-scale identification and quantification of proteins, as well as the characterisation of their complexes and post-translational modifications. This review describes the instrumentation used for proteomics research. It presents an overview of the current applications of mass spectrometry-based proteomics to the cellular and molecular life sciences, and discusses challenges that exist for research in the future.Mesh：
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Year: 2005 PMID： 15868409 DOI： 10.1007/s00018-005-5006-6
Source DB: PubMed Journal: Cell Mol Life Sci ISSN： 1420-682X Impact factor: 9.261