| Literature DB >> 15858142 |
Norifumi Naka1, Susumu Joyama, Yoshitane Tsukamoto, Kiyoko Yoshioka, Nobuyuki Hashimoto, Takeshi Ujiiye, Tsukasa Hayashi, Masako Kawase, Masayuki Mano, Shingo Ishiguro, Akira Myoui, Takafumi Ueda, Hideki Yoshikawa, Nobuhito Araki, Kazuyuki Itoh.
Abstract
The SSX family proteins have been considered new members of the cancer/testis antigens because of the restricted expression in testis among normal tissues and the activation in a wide range of cancers. Thus, they would be potential molecular targets for immunotherapeutic strategies. We have developed a competitive nucleic acid sequence-based amplification (NASBA) assay to analyze SSX mRNA expression in 211 bone and soft tissue tumors. The copy numbers of SSX mRNA per mug of total RNA in tumor tissues were widely distributed, ranging logarithmically from 0.6 to 6.6. We found that malignant tumors showed significantly higher expression of SSX mRNA than benign tumors (P < 0.0001). Further, SSX mRNA expression in stage III tumors was significantly higher than that in stage I or II tumors (P < 0.005). This NASBA assay was also more sensitive compared to immunohistochemistry using newly affinity-purified polyclonal antibody against SSX. Collectively, these results suggest that the SSX quantitative NASBA assay could provide useful information to select eligible patients for SSX-specific cancer vaccines.Entities:
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Year: 2005 PMID： 15858142 PMCID： PMC1867521 DOI： 10.1016/S1525-1578(10)60545-4
Source DB: PubMed Journal: J Mol Diagn ISSN： 1525-1578 Impact factor: 5.568