| Literature DB >> 15823603 |
Kate Porter1, Petra Kukkaro, Jaana K H Bamford, Carolyn Bath, Hanna M Kivelä, Mike L Dyall-Smith, Dennis H Bamford.
Abstract
A novel halovirus, SH1, with a spherical morphology is described. Isolated from a hypersaline lake, SH1 is divalent, producing clear plaques on Haloarcula hispanica and a natural Halorubrum isolate. Single-step growth curves gave a latent period of 5-6 h and a burst size of around 200 PFU/cell. The host can differentiate to form tight clusters of thick cell-walled forms, and these were shown to be resistant to infection. Purified virions had no visible tail, were about 70 nm in diameter, and displayed a fragile outer capsid layer, possibly with an underlying membrane component. The structural proteins of the virion were analyzed by SDS-PAGE and several were found to be cross-linked, forming protein complexes. The genome was linear, dsDNA, of approximately 30 kb in length. This morphology and linear genome are features not observed in any other euryarchaeal viruses, but have properties similar to the bacterial virus PRD1.Entities:
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Year: 2005 PMID： 15823603 DOI： 10.1016/j.virol.2005.01.043
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616