Irina V Larina1, B Mark Evers, Rinat O Esenaliev. 1. Center for Biomedical Engineering, Department of Neuroscience and Cell Biology, The University of Texas Medical Branch, Galveston, Texas 77555, USA.
Abstract
BACKGROUND: Lack of efficient drug and gene delivery is one of the major problems of cancer chemo- and bio-therapy. This work is focused on optimization of ultrasound-induced delivery of model macromolecular anti-cancer drugs and DNA in human cancer cells. MATERIALS AND METHODS: FITC dextrans simulated antisense oligonucleotides (10 kDa), antibodies (70 kDa), and genes (2000 kDa). Optimization of ultrasound frequency, intensity, duty cycle, time of irradiation, and concentration of Optison (ultrasound contrast agent) was performed. RESULTS: Optimal parameters provided in the MCF7 cell line 73.5+/-3.3%, 72.7+/-0.9%, and 62.7+/-2.1% delivery of 10-kDa, 70-kDa, and 2000-kDa macromolecules, respectively, 36.7+/-4.9% of cell transfection, while dead cell count was only 13.5+/-1.6%. Statistically significant drug delivery and transfection was obtained in all tested cell lines. CONCLUSION: These results suggest that optimized treatment parameters provide efficient drug and gene delivery in cancer cells and could be used for further in vivo and in vitro experiments.
BACKGROUND: Lack of efficient drug and gene delivery is one of the major problems of cancer chemo- and bio-therapy. This work is focused on optimization of ultrasound-induced delivery of model macromolecular anti-cancer drugs and DNA in humancancer cells. MATERIALS AND METHODS:FITC dextrans simulated antisense oligonucleotides (10 kDa), antibodies (70 kDa), and genes (2000 kDa). Optimization of ultrasound frequency, intensity, duty cycle, time of irradiation, and concentration of Optison (ultrasound contrast agent) was performed. RESULTS: Optimal parameters provided in the MCF7 cell line 73.5+/-3.3%, 72.7+/-0.9%, and 62.7+/-2.1% delivery of 10-kDa, 70-kDa, and 2000-kDa macromolecules, respectively, 36.7+/-4.9% of cell transfection, while dead cell count was only 13.5+/-1.6%. Statistically significant drug delivery and transfection was obtained in all tested cell lines. CONCLUSION: These results suggest that optimized treatment parameters provide efficient drug and gene delivery in cancer cells and could be used for further in vivo and in vitro experiments.
Authors: Robyn K Schlicher; Joshua D Hutcheson; Harish Radhakrishna; Robert P Apkarian; Mark R Prausnitz Journal: Ultrasound Med Biol Date: 2010-04 Impact factor: 2.998
Authors: Adelaide Greco; Altomare Di Benedetto; Candace M Howard; Sarah Kelly; Rounak Nande; Yulia Dementieva; Michele Miranda; Arturo Brunetti; Marco Salvatore; Luigi Claudio; Devanand Sarkar; Paul Dent; David T Curiel; Paul B Fisher; Pier P Claudio Journal: Mol Ther Date: 2009-11-03 Impact factor: 11.454
Authors: Ghaleb A Husseini; Dana Stevenson-Abouelnasr; William G Pitt; Khaled T Assaleh; Lujein O Farahat; Jalal Fahadi Journal: Colloids Surf A Physicochem Eng Asp Date: 2010-04-20 Impact factor: 4.539