Literature DB >> 15812058

Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.

Morgan Guilbaud1, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Hervé Prévost, Xavier Dousset.   

Abstract

A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.

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Year:  2005        PMID: 15812058      PMCID: PMC1082516          DOI: 10.1128/AEM.71.4.2190-2194.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  20 in total

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2.  PCR detection of Listeria monocytogenes in 'gravad' rainbow trout.

Authors:  H Ericsson; P Stålhandske
Journal:  Int J Food Microbiol       Date:  1997-04-15       Impact factor: 5.277

3.  Behaviour of L. monocytogenes in an artificially made biofilm of a nisin-producing strain of Lactococcus lactis.

Authors:  V Leriche; D Chassaing; B Carpentier
Journal:  Int J Food Microbiol       Date:  1999-10-15       Impact factor: 5.277

4.  Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation.

Authors:  D Djordjevic; M Wiedmann; L A McLandsborough
Journal:  Appl Environ Microbiol       Date:  2002-06       Impact factor: 4.792

5.  Comparative evaluation of adhesion and biofilm formation of different Listeria monocytogenes strains.

Authors:  M S Chae; H Schraft
Journal:  Int J Food Microbiol       Date:  2000-12-05       Impact factor: 5.277

6.  Persistent Listeria monocytogenes strains show enhanced adherence to food contact surface after short contact times.

Authors:  J M Lundén; M K Miettinen; T J Autio; H J Korkeala
Journal:  J Food Prot       Date:  2000-09       Impact factor: 2.077

7.  Application of 5'-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk.

Authors:  H K Nogva; K Rudi; K Naterstad; A Holck; D Lillehaug
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

8.  Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay.

Authors:  I Hein; D Klein; A Lehner; A Bubert; E Brandl; M Wagner
Journal:  Res Microbiol       Date:  2001 Jan-Feb       Impact factor: 3.992

9.  Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis.

Authors:  J J Tudor; L Marri; P J Piggot; L Daneo-Moore
Journal:  Infect Immun       Date:  1990-03       Impact factor: 3.441

10.  Rapid enumeration of Listeria monocytogenes in artificially contaminated cabbage using real-time polymerase chain reaction.

Authors:  Angela J Hough; Sally-Ann Harbison; Marion G Savill; Laurence D Melton; Graham Fletcher
Journal:  J Food Prot       Date:  2002-08       Impact factor: 2.077

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  12 in total

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Journal:  J Ind Microbiol Biotechnol       Date:  2010-05-11       Impact factor: 3.346

2.  Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR.

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Review 3.  Unraveling microbial biofilms of importance for food microbiology.

Authors:  Lizziane Kretli Winkelströter; Fernanda Barbosa dos Reis Teixeira; Eliane Pereira Silva; Virgínia Farias Alves; Elaine Cristina Pereira De Martinis
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4.  Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains.

Authors:  Li Wang; Yue Li; Jin Chu; Zhenbo Xu; Qingping Zhong
Journal:  Mol Biol Rep       Date:  2011-05-14       Impact factor: 2.316

5.  Development of defined microbial population standards using fluorescence activated cell sorting for the absolute quantification of S. aureus using real-time PCR.

Authors:  Alice Martinon; Ultan P Cronin; Martin G Wilkinson
Journal:  Mol Biotechnol       Date:  2012-01       Impact factor: 2.695

Review 6.  Options and Limitations in Clinical Investigation of Bacterial Biofilms.

Authors:  Maria Magana; Christina Sereti; Anastasios Ioannidis; Courtney A Mitchell; Anthony R Ball; Emmanouil Magiorkinis; Stylianos Chatzipanagiotou; Michael R Hamblin; Maria Hadjifrangiskou; George P Tegos
Journal:  Clin Microbiol Rev       Date:  2018-04-04       Impact factor: 26.132

7.  Diagnosis of Listeria monocytogenes meningoencephalitis by real-time PCR for the hly gene.

Authors:  Alban Le Monnier; Eric Abachin; Jean-Luc Beretti; Patrick Berche; Samer Kayal
Journal:  J Clin Microbiol       Date:  2011-09-14       Impact factor: 5.948

8.  Colorimetric assay for biofilms in wet processing conditions.

Authors:  Judy W Arnold
Journal:  J Ind Microbiol Biotechnol       Date:  2008-08-20       Impact factor: 3.346

9.  Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells.

Authors:  Y Pan; F Breidt
Journal:  Appl Environ Microbiol       Date:  2007-10-12       Impact factor: 4.792

Review 10.  Waterborne pathogens: detection methods and challenges.

Authors:  Flor Yazmín Ramírez-Castillo; Abraham Loera-Muro; Mario Jacques; Philippe Garneau; Francisco Javier Avelar-González; Josée Harel; Alma Lilián Guerrero-Barrera
Journal:  Pathogens       Date:  2015-05-21
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