Literature DB >> 21573733

Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains.

Li Wang1, Yue Li, Jin Chu, Zhenbo Xu, Qingping Zhong.   

Abstract

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65 °C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays.

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Year:  2011        PMID： 21573733     DOI： 10.1007/s11033-011-0757-7

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  21 in total

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5.  Application of 5'-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk.

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9.  Integron-bearing methicillin-resistant coagulase-negative staphylococci in South China, 2001-2004.

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  29 in total

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7.  Establishment and application of a rapid visual detection method for Listeria monocytogenes based on polymerase spiral reaction (PSR).

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8.  Characterization on gut microbiome of PCOS rats and its further design by shifts in high-fat diet and dihydrotestosterone induction in PCOS rats.

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9.  "One-step" characterization platform for pathogenic genetics of Staphylococcus aureus.

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10.  One-Day Molecular Detection of Salmonella and Campylobacter in Chicken Meat: A Pilot Study.

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Journal:  Foods       Date:  2021-05-19
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