Literature DB >> 15808232

Solution NMR spin relaxation methods for characterizing chemical exchange in high-molecular-weight systems.

Arthur G Palmer1, Michael J Grey, Chunyu Wang.   

Abstract

Transverse relaxation optimized NMR spectroscopy (TROSY) techniques for (1)H-(15)N backbone amide moieties and for (13)CH(3) methyl groups have permitted the development of Hahn spin echo and Carr-Purcell-Meiboom-Gill (CPMG) experiments for characterizing chemical exchange kinetic phenomena on microsecond-millisecond time scales in proteins with molecular masses >50 kDa. This chapter surveys the theoretical bases for TROSY in spin systems subject to chemical exchange linebroadening, the experimental methods that have been developed to quantitatively characterize chemical exchange in large proteins, and the emerging applications to triose phosphate isomerase, hemoglobin, and malate synthase G, with molecular masses ranging from 54 to 82 kDa.

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Year:  2005        PMID： 15808232     DOI： 10.1016/S0076-6879(05)94018-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  33 in total

1.  GUARDD: user-friendly MATLAB software for rigorous analysis of CPMG RD NMR data.

Authors:  Ian R Kleckner; Mark P Foster
Journal:  J Biomol NMR       Date:  2011-12-11       Impact factor: 2.835

2.  Determining protein dynamics from ¹⁵N relaxation data by using DYNAMICS.

Authors:  David Fushman
Journal:  Methods Mol Biol       Date:  2012

3.  Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.

Authors:  Hak Jun Kim; Stanley C Howell; Wade D Van Horn; Young Ho Jeon; Charles R Sanders
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2009-11-01       Impact factor: 9.795

Review 4.  Solution NMR of large molecules and assemblies.

Authors:  Mark P Foster; Craig A McElroy; Carlos D Amero
Journal:  Biochemistry       Date:  2007-01-16       Impact factor: 3.162

5.  Backbone dynamics in an intramolecular prolylpeptide-SH3 complex from the diphtheria toxin repressor, DtxR.

Authors:  Nilakshee Bhattacharya; Myunggi Yi; Huan-Xiang Zhou; Timothy M Logan
Journal:  J Mol Biol       Date:  2007-10-31       Impact factor: 5.469

6.  Intramolecular domain-domain association/dissociation and phosphoryl transfer in the mannitol transporter of Escherichia coli are not coupled.

Authors:  Jeong-Yong Suh; Junji Iwahara; G Marius Clore
Journal:  Proc Natl Acad Sci U S A       Date:  2007-02-21       Impact factor: 11.205

Review 7.  Relaxation dispersion NMR spectroscopy as a tool for detailed studies of protein folding.

Authors:  Philipp Neudecker; Patrik Lundström; Lewis E Kay
Journal:  Biophys J       Date:  2009-03-18       Impact factor: 4.033

Review 8.  Chemical exchange in biomacromolecules: past, present, and future.

Authors:  Arthur G Palmer
Journal:  J Magn Reson       Date:  2014-04       Impact factor: 2.229

9.  Measurement of signs of chemical shift differences between ground and excited protein states: a comparison between H(S/M)QC and R1rho methods.

Authors:  Renate Auer; D Flemming Hansen; Philipp Neudecker; Dmitry M Korzhnev; D Ranjith Muhandiram; Robert Konrat; Lewis E Kay
Journal:  J Biomol NMR       Date:  2009-12-22       Impact factor: 2.835

10.  Measuring 13Cbeta chemical shifts of invisible excited states in proteins by relaxation dispersion NMR spectroscopy.

Authors:  Patrik Lundström; Hong Lin; Lewis E Kay
Journal:  J Biomol NMR       Date:  2009-05-16       Impact factor: 2.835
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