| Literature DB >> 15704127 |
Christoph Grunau1, Cecilia Sanchez, Melanie Ehrlich, Pierre van der Bruggen, Winfried Hindermann, Carmen Rodriguez, Sophie Krieger, Louis Dubeau, Emerich Fiala, Albertina De Sario.
Abstract
The BAGE (B melanoma antigens) sequence family contains 15 nearly identical sequences that are in the juxtacentromeric regions of chromosomes 9, 13, 18, and 21. BAGE loci are expressed in male germ tissue and in a high percentage of cancers and cancer cell lines. We analyzed the DNA methylation state of the sequences in or near the promoters of the BAGE loci by a quantitative bisulfite and PCR-based assay (multiplex COBRA) using MboI and HphI in 18 somatic tissue samples, 4 testis and 4 sperm samples, and 48 tumors and tumor cell lines. In 94% of the control somatic tissue samples, DNA was highly methylated in the analyzed regions. In contrast, 98% of tumor DNA samples displayed hypomethylation. Also, DNA from testes and sperm was hypomethylated in at least one of the BAGE loci. BAGE transcripts were observed in only 47% of the analyzed tumor samples. Consequently, we propose BAGE hypomethylation as a new, highly informative epigenetic biomarker for the diagnosis of cancer, whose hypomethylation in cancer may be causally related to that of juxtacentromeric satellite DNA. Copyright 2005 Wiley-Liss, Inc.Entities:
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Year: 2005 PMID： 15704127 DOI： 10.1002/gcc.20155
Source DB: PubMed Journal: Genes Chromosomes Cancer ISSN： 1045-2257 Impact factor: 5.006