Literature DB >> 15656605

Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases.

Alexander Heckel1, Günter Mayer.   

Abstract

"Caged" derivatives of a 15 nucleotide ssDNA anti-thrombin aptamer have been synthesized in which thymidine nucleotides are modified with photolabile protecting groups. One caged thymidine in a key location is enough to completely mask the aptamer's function in respect to their affinity for thrombin and their inhibition of the blood clotting cascade. With light (366 nm) the caging group can be removed, yielding the unmodified active aptamer.

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Year:  2005        PMID： 15656605     DOI： 10.1021/ja043285e

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  23 in total

1.  Photochemical DNA activation.

Authors:  Hrvoje Lusic; Douglas D Young; Mark O Lively; Alexander Deiters
Journal:  Org Lett       Date:  2007-04-21       Impact factor: 6.005

2.  Light-dependent RNA interference with nucleobase-caged siRNAs.

Authors:  Vera Mikat; Alexander Heckel
Journal:  RNA       Date:  2007-10-19       Impact factor: 4.942

3.  Light-triggered polymerase chain reaction.

Authors:  Douglas D Young; Wesleigh F Edwards; Hrvoje Lusic; Mark O Lively; Alexander Deiters
Journal:  Chem Commun (Camb)       Date:  2007-11-14       Impact factor: 6.222

4.  Light-regulated RNA-small molecule interactions.

Authors:  Douglas D Young; Alexander Deiters
Journal:  Chembiochem       Date:  2008-05-23       Impact factor: 3.164

5.  Light-activated deoxyguanosine: photochemical regulation of peroxidase activity.

Authors:  Hrvoje Lusic; Mark O Lively; Alexander Deiters
Journal:  Mol Biosyst       Date:  2008-04-08

6.  In situ assembly of macromolecular complexes triggered by light.

Authors:  Christian Grunwald; Katrin Schulze; Annett Reichel; Victor U Weiss; Dieter Blaas; Jacob Piehler; Karl-Heinz Wiesmüller; Robert Tampé
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-03       Impact factor: 11.205

Review 7.  Optochemical Control of Biological Processes in Cells and Animals.

Authors:  Nicholas Ankenbruck; Taylor Courtney; Yuta Naro; Alexander Deiters
Journal:  Angew Chem Int Ed Engl       Date:  2018-02-01       Impact factor: 15.336

8.  Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.

Authors:  XinJing Tang; Meng Su; LiLi Yu; Cong Lv; Jie Wang; ZhongJin Li
Journal:  Nucleic Acids Res       Date:  2010-02-17       Impact factor: 16.971

9.  Light-activated RNA interference using double-stranded siRNA precursors modified using a remarkable regiospecificity of diazo-based photolabile groups.

Authors:  Samit Shah; Piyush K Jain; Ashish Kala; Dipu Karunakaran; Simon H Friedman
Journal:  Nucleic Acids Res       Date:  2009-05-28       Impact factor: 16.971

10.  Dependence of aptamer activity on opposed terminal extensions: improvement of light-regulation efficiency.

Authors:  Maximilian C R Buff; Florian Schäfer; Bernhard Wulffen; Jens Müller; Bernd Pötzsch; Alexander Heckel; Günter Mayer
Journal:  Nucleic Acids Res       Date:  2009-12-08       Impact factor: 16.971
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