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Literature DB >> 15642946

The fluorescence resonance energy transfer (FRET) gate: a time-resolved study.

Qing-Hua Xu¹, Shu Wang, Dmitry Korystov, Alexander Mikhailovsky, Guillermo C Bazan, Daniel Moses, Alan J Heeger.

Abstract

The two-step energy-transfer process in a self-assembled complex comprising a cationic conjugated polymer (CCP) and a dsDNA is investigated by using pump-dump-emission spectroscopy and time-correlated single-photon counting; energy is transferred from the CCP to an ethidium bromide (EB) molecule intercalated into the dsDNA through a fluorescein molecule linked to one terminus of the DNA. Time-dependent anisotropy measurements indicate that the inefficient direct energy transfer from the CCP to the intercalated EB results from the near orthogonality of their transition moments. These measurements also show that the transition moment of the fluorescein spans a range of angular distributions and lies between that of the CCP and EB. Consequently, the fluorescein acts as a fluorescence resonance energy-transfer gate to relay the excitation energy from the CCP to the EB.

Entities: Chemical

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Year: 2005 PMID： 15642946 PMCID： PMC545565 DOI： 10.1073/pnas.0408568102

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Effect of chromophore-charge distance on the energy transfer properties of water-soluble conjugated oligomers.

Authors: Bin Liu; Brent S Gaylord; Shu Wang; Guillermo C Bazan
Journal: J Am Chem Soc Date: 2003-06-04 Impact factor: 15.419

2. Interchain Excitations in Conjugated Polymers.

Authors:
Journal: Phys Rev Lett Date: 1995-09-04 Impact factor: 9.161

3. Interaction of ethidium bromide with whole and selectively deproteinized deoxynucleoproteins from calf thymus.

Authors: L M Angerer; E N Moudrianakis
Journal: J Mol Biol Date: 1972-02-14 Impact factor: 5.469

4. Fluorescence emission of ethidium bromide intercalated in defined DNA duplexes: evaluation of hydrodynamics components.

Authors: J Duhamel; J Kanyo; G Dinter-Gottlieb; P Lu
Journal: Biochemistry Date: 1996-12-24 Impact factor: 3.162

5. Precursor states for charge carrier generation in conjugated polymers probed by ultrafast spectroscopy.

Authors: J G Müller; U Lemmer; J Feldmann; U Scherf
Journal: Phys Rev Lett Date: 2002-03-20 Impact factor: 9.161

6. DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes.

Authors: Brent S Gaylord; Alan J Heeger; Guillermo C Bazan
Journal: Proc Natl Acad Sci U S A Date: 2002-08-07 Impact factor: 11.205

7. Fluorescein provides a resonance gate for FRET from conjugated polymers to DNA intercalated dyes.

Authors: Shu Wang; Brent S Gaylord; Guillermo C Bazan
Journal: J Am Chem Soc Date: 2004-05-05 Impact factor: 15.419

8. Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions.

Authors: Qing-Hua Xu; Brent S Gaylord; Shu Wang; Guillermo C Bazan; Daniel Moses; Alan J Heeger
Journal: Proc Natl Acad Sci U S A Date: 2004-07-28 Impact factor: 11.205

9. A molecular dynamics simulation study of oriented DNA with polyamine and sodium counterions: diffusion and averaged binding of water and cations.

Authors: Nikolay Korolev; Alexander P Lyubartsev; Aatto Laaksonen; Lars Nordenskiöld
Journal: Nucleic Acids Res Date: 2003-10-15 Impact factor: 16.971

The fluorescence resonance energy transfer (FRET) gate: a time-resolved study.

1. Effect of chromophore-charge distance on the energy transfer properties of water-soluble conjugated oligomers.

2. Interchain Excitations in Conjugated Polymers.

3. Interaction of ethidium bromide with whole and selectively deproteinized deoxynucleoproteins from calf thymus.

4. Fluorescence emission of ethidium bromide intercalated in defined DNA duplexes: evaluation of hydrodynamics components.

5. Precursor states for charge carrier generation in conjugated polymers probed by ultrafast spectroscopy.

6. DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes.

7. Fluorescein provides a resonance gate for FRET from conjugated polymers to DNA intercalated dyes.

8. Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions.

9. A molecular dynamics simulation study of oriented DNA with polyamine and sodium counterions: diffusion and averaged binding of water and cations.

10. Shape-adaptable water-soluble conjugated polymers.

1. Cooperation of Escherichia coli Hfq hexamers in DsrA binding.

2. Fluorescence resonance energy transfer in polydiacetylene liposomes.

3. On the binding of cationic, water-soluble conjugated polymers to DNA: electrostatic and hydrophobic interactions.

4. Energy Transfer in a Nanoscale Multichromophoric System: Fluorescent Dye-Doped Conjugated Polymer Nanoparticles.

Review 5. Highly fluorescent semiconducting polymer dots for biology and medicine.