Literature DB >> 20058862

On the binding of cationic, water-soluble conjugated polymers to DNA: electrostatic and hydrophobic interactions.

Fan Xia1, Xiaolei Zuo, Renqiang Yang, Yi Xiao, Di Kang, Alexis Vallée-Bélisle, Xiong Gong, Alan J Heeger, Kevin W Plaxco.   

Abstract

Water-soluble, cationic conjugated polymer binds single-stranded DNA with higher affinity than it binds double-stranded or otherwise "folded" DNA. This stronger binding results from the greater hydrophobicity of single-stranded DNA. Upon reducing the strength of the hydrophobic interactions, the electrostatic attraction becomes the important interaction that regulates the binding between the water-soluble conjugated polymer and DNA. The different affinities between the cationic conjugated polymer and various forms of DNA (molecular beacons and its open state; single-stranded DNA and double-stranded DNA and single-stranded DNA and complex DNA folds) can be used to design a variety of biosensors.

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Year:  2010        PMID： 20058862      PMCID： PMC2846820          DOI： 10.1021/ja908890q

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  25 in total

1.  Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules.

Authors:  M Han; X Gao; J Z Su; S Nie
Journal:  Nat Biotechnol       Date:  2001-07       Impact factor: 54.908

2.  A sensitive and homogeneous SNP detection using cationic conjugated polymers.

Authors:  Xinrui Duan; Zhengping Li; Fang He; Shu Wang
Journal:  J Am Chem Soc       Date:  2007-03-20       Impact factor: 15.419

3.  Ion motion in conjugated polyelectrolyte electron transporting layers.

Authors:  Corey Hoven; Renqiang Yang; Andres Garcia; Alan J Heeger; Thuc-Quyen Nguyen; Guillermo C Bazan
Journal:  J Am Chem Soc       Date:  2007-08-17       Impact factor: 15.419

4.  Visual cocaine detection with gold nanoparticles and rationally engineered aptamer structures.

Authors:  Juan Zhang; Lihua Wang; Dun Pan; Shiping Song; Freddy Y C Boey; Hua Zhang; Chunhai Fan
Journal:  Small       Date:  2008-08       Impact factor: 13.281

5.  Colorimetric and fluorometric detection of nucleic acids using cationic polythiophene derivatives.

Authors:  Hoang-Anh Ho; Maurice Boissinot; Michel G Bergeron; Geneviève Corbeil; Kim Doré; Denis Boudreau; Mario Leclerc
Journal:  Angew Chem Int Ed Engl       Date:  2002-05-03       Impact factor: 15.336

6.  Molecular beacons: probes that fluoresce upon hybridization.

Authors:  S Tyagi; F R Kramer
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908

7.  High-efficiency fluorescence quenching of conjugated polymers by proteins.

Authors:  Chunhai Fan; Kevin W Plaxco; Alan J Heeger
Journal:  J Am Chem Soc       Date:  2002-05-22       Impact factor: 15.419

Review 8.  Optimizing the cationic conjugated polymer-sensitized fluorescent signal of dye labeled oligonucleotide for biosensor applications.

Authors:  Kan-Yi Pu; Bin Liu
Journal:  Biosens Bioelectron       Date:  2008-07-26       Impact factor: 10.618

9.  Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions.

Authors:  Qing-Hua Xu; Brent S Gaylord; Shu Wang; Guillermo C Bazan; Daniel Moses; Alan J Heeger
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-28       Impact factor: 11.205

10.  High specificity, electrochemical sandwich assays based on single aptamer sequences and suitable for the direct detection of small-molecule targets in blood and other complex matrices.

Authors:  Xiaolei Zuo; Yi Xiao; Kevin W Plaxco
Journal:  J Am Chem Soc       Date:  2009-05-27       Impact factor: 15.419
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  5 in total

1.  Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.

Authors:  Fan Xia; Xiaolei Zuo; Renqiang Yang; Yi Xiao; Di Kang; Alexis Vallée-Bélisle; Xiong Gong; Jonathan D Yuen; Ben B Y Hsu; Alan J Heeger; Kevin W Plaxco
Journal:  Proc Natl Acad Sci U S A       Date:  2010-06-01       Impact factor: 11.205

2.  Utilizing Intrinsic Properties of Polyaniline to Detect Nucleic Acid Hybridization through UV-Enhanced Electrostatic Interaction.

Authors:  Partha Pratim Sengupta; Jared N Gloria; Dahlia N Amato; Douglas V Amato; Derek L Patton; Beddhu Murali; Alex S Flynt
Journal:  Biomacromolecules       Date:  2015-09-29       Impact factor: 6.988

3.  Fluorescence protection assay: a novel homogeneous assay platform toward development of aptamer sensors for protein detection.

Authors:  Hong-Qi Wang; Zhan Wu; Li-Juan Tang; Ru-Qin Yu; Jian-Hui Jiang
Journal:  Nucleic Acids Res       Date:  2011-07-08       Impact factor: 16.971

4.  A novel property of DNA - as a bioflotation reagent in mineral processing.

Authors:  Balasubramanian Vasanthakumar; Honnavar Ravishankar; Sankaran Subramanian
Journal:  PLoS One       Date:  2012-07-02       Impact factor: 3.240

5.  Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays.

Authors:  Ji-Eun Jeong; Boram Kim; Shinjae Woo; Sungu Hwang; Guillermo C Bazan; Han Young Woo
Journal:  Chem Sci       Date:  2015-01-07       Impact factor: 9.825
  5 in total

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