Literature DB >> 12167673

DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes.

Brent S Gaylord1, Alan J Heeger, Guillermo C Bazan.   

Abstract

The light-harvesting properties of cationic conjugated polymers are used to sensitize the emission of a dye on a specific peptide nucleic acid (PNA) sequence for the purpose of homogeneous, "real-time" DNA detection. Signal transduction is controlled by hybridization of the neutral PNA probe and the negative DNA target. Electrostatic interactions bring the hybrid complex and cationic polymer within distances required for Förster energy transfer. Conjugated polymer excitation provides fluorescein emission >25 times higher than that obtained by exciting the dye, allowing detection of target DNA at concentrations of 10 pM with a standard fluorometer. A simple and highly sensitive assay with optical amplification that uses the improved hybridization behavior of PNA/DNA complexes is thus demonstrated.

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Year:  2002        PMID： 12167673      PMCID： PMC123191          DOI： 10.1073/pnas.162375999

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer.

Authors:  L Chen; D W McBranch; H L Wang; R Helgeson; F Wudl; D G Whitten
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-26       Impact factor: 11.205

Review 2.  Single nucleotide polymorphisms and the future of genetic epidemiology.

Authors:  N J Schork; D Fallin; J S Lanchbury
Journal:  Clin Genet       Date:  2000-10       Impact factor: 4.438

Review 3.  From DNA biosensors to gene chips.

Authors:  J Wang
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

Review 4.  PNA for rapid microbiology.

Authors:  Henrik Stender; Mark Fiandaca; Jens J Hyldig-Nielsen; James Coull
Journal:  J Microbiol Methods       Date:  2002-01       Impact factor: 2.363

Review 5.  Applications of peptide nucleic acids.

Authors:  P E Nielsen
Journal:  Curr Opin Biotechnol       Date:  1999-02       Impact factor: 9.740

6.  A formula for thermal stability (Tm) prediction of PNA/DNA duplexes.

Authors:  U Giesen; W Kleider; C Berding; A Geiger; H Orum; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1998-11-01       Impact factor: 16.971

7.  Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA.

Authors:  A Castro; J G Williams
Journal:  Anal Chem       Date:  1997-10-01       Impact factor: 6.986

Review 8.  Tumor targeting with surface-shielded ligand--polycation DNA complexes.

Authors:  R Kircheis; T Blessing; S Brunner; L Wightman; E Wagner
Journal:  J Control Release       Date:  2001-05-14       Impact factor: 9.776

9.  Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes.

Authors:  K V Balakin; V A Korshun; I I Mikhalev; G V Maleev; A D Malakhov; I A Prokhorenko
Journal:  Biosens Bioelectron       Date:  1998-10-01       Impact factor: 10.618

10.  Polyelectrolyte vectors for gene delivery: influence of cationic polymer on biophysical properties of complexes formed with DNA.

Authors:  M A Wolfert; P R Dash; O Nazarova; D Oupicky; L W Seymour; S Smart; J Strohalm; K Ulbrich
Journal:  Bioconjug Chem       Date:  1999 Nov-Dec       Impact factor: 4.774
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  37 in total

1.  Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA.

Authors:  Chunhai Fan; Kevin W Plaxco; Alan J Heeger
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-16       Impact factor: 11.205

2.  Self-assembly of synthetic peptides control conformation and optical properties of a zwitterionic polythiophene derivative.

Authors:  K Peter R Nilsson; Johan Rydberg; Lars Baltzer; Olle Inganäs
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-19       Impact factor: 11.205

3.  Foldamers with hybrid biological and synthetic sequences as selective DNA fluorescent probes.

Authors:  Wei Wang; Wei Wan; Andrew Stachiw; Alexander D Q Li
Journal:  Biochemistry       Date:  2005-08-16       Impact factor: 3.162

4.  Investigation of a fluorescence signal amplification mechanism used for the direct molecular detection of nucleic acids.

Authors:  Kim Doré; Mario Leclerc; Denis Boudreau
Journal:  J Fluoresc       Date:  2006-05-02       Impact factor: 2.217

5.  SNP detection using peptide nucleic acid probes and conjugated polymers: applications in neurodegenerative disease identification.

Authors:  Brent S Gaylord; Michelle R Massie; Stuart C Feinstein; Guillermo C Bazan
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-23       Impact factor: 11.205

6.  Efficient quenching of oligomeric fluorophores on a DNA backbone.

Authors:  James N Wilson; Yin Nah Teo; Eric T Kool
Journal:  J Am Chem Soc       Date:  2007-11-21       Impact factor: 15.419

7.  Effect of molecular crowding on the response of an electrochemical DNA sensor.

Authors:  Francesco Ricci; Rebecca Y Lai; Alan J Heeger; Kevin W Plaxco; James J Sumner
Journal:  Langmuir       Date:  2007-05-09       Impact factor: 3.882

8.  Formation and characterization of stable fluorescent complexes between neutral conjugated polymers and cyclodextrins.

Authors:  Maria José Martínez-Tomé; Rocío Esquembre; Ricardo Mallavia; C Reyes Mateo
Journal:  J Fluoresc       Date:  2012-09-20       Impact factor: 2.217

9.  Fluorescence resonance energy transfer in polydiacetylene liposomes.

Authors:  Xuelian Li; Shelton Matthews; Punit Kohli
Journal:  J Phys Chem B       Date:  2008-09-25       Impact factor: 2.991

10.  Amplified fluorescence sensing of protease activity with conjugated polyelectrolytes.

Authors:  Mauricio R Pinto; Kirk S Schanze
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-10       Impact factor: 11.205
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