Literature DB >> 15615040

Cy3B: improving the performance of cyanine dyes.

Michael Cooper1, Andreas Ebner, Mark Briggs, Miles Burrows, Nicholas Gardner, Robert Richardson, Richard West.   

Abstract

The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.

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Year:  2004        PMID: 15615040     DOI: 10.1023/b:jofl.0000016286.62641.59

Source DB:  PubMed          Journal:  J Fluoresc        ISSN: 1053-0509            Impact factor:   2.217


  12 in total

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4.  1536-well assay plates: when do they make sense?

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Review 5.  Image-based screening of signal transduction assays.

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6.  Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin.

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Journal:  Bioconjug Chem       Date:  2000 Sep-Oct       Impact factor: 4.774

7.  Influence of coupling method on the luminescence properties, coupling efficiency, and binding affinity of antibodies labeled with europium (III) chelates.

Authors:  H Karşilayan; I Hemmilä; H Takalo; A Toivonen; K Pettersson; T Lövgren; V M Mukkala
Journal:  Bioconjug Chem       Date:  1997 Jan-Feb       Impact factor: 4.774

8.  Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 kinases, 1 protease, and 1 phosphatase.

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9.  Characterization of fluorescein-oligonucleotide conjugates and measurement of local electrostatic potential.

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Journal:  Biopolymers       Date:  1998-12       Impact factor: 2.505

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  29 in total

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Review 4.  smFRET studies of the 'encounter' complexes and subsequent intermediate states that regulate the selectivity of ligand binding.

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6.  Riboswitch structure and dynamics by smFRET microscopy.

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Review 8.  Productive Manipulation of Cyanine Dye π-Networks.

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9.  Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures.

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Review 10.  Fluorescence anisotropy (polarization): from drug screening to precision medicine.

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