PURPOSE: To investigate whether removal of extraneous cells and immotile spermatozoa from a sperm preparation by density gradient centrifugation could help to maintain normal spermatozoa in a viable state and retain their deoxyribonucleic acid integrity. METHODS: Sperm motility was assessed on a daily basis in aliquots of neat semen, extended semen, and spermatozoa prepared on a PureSperm density gradient. At the same time, aliquots of each sperm sample were preserved for TUNEL assay and nick translation. RESULTS: Spermatozoa prepared using density gradient centrifugation survived three times as long as spermatozoa in neat semen or in extended semen. Both deoxyribonucleic acid integrity and sperm motility were retained in the gradient preparations. CONCLUSIONS: Preparing spermatozoa by density gradient centrifugation is advantageous in prolonging sperm survival and maintaining deoxyribonucleic acid integrity, presumably by removing sources of reactive oxygen species. Stored spermatozoa could be used for a second attempt at fertilization if oocyte immaturity was suspected.
PURPOSE: To investigate whether removal of extraneous cells and immotile spermatozoa from a sperm preparation by density gradient centrifugation could help to maintain normal spermatozoa in a viable state and retain their deoxyribonucleic acid integrity. METHODS: Sperm motility was assessed on a daily basis in aliquots of neat semen, extended semen, and spermatozoa prepared on a PureSperm density gradient. At the same time, aliquots of each sperm sample were preserved for TUNEL assay and nick translation. RESULTS: Spermatozoa prepared using density gradient centrifugation survived three times as long as spermatozoa in neat semen or in extended semen. Both deoxyribonucleic acid integrity and sperm motility were retained in the gradient preparations. CONCLUSIONS: Preparing spermatozoa by density gradient centrifugation is advantageous in prolonging sperm survival and maintaining deoxyribonucleic acid integrity, presumably by removing sources of reactive oxygen species. Stored spermatozoa could be used for a second attempt at fertilization if oocyte immaturity was suspected.
Authors: Erica T Y Leung; Cheuk-Lun Lee; Xinyi Tian; Kevin K W Lam; Raymond H W Li; Ernest H Y Ng; William S B Yeung; Philip C N Chiu Journal: Nat Rev Urol Date: 2021-11-05 Impact factor: 14.432
Authors: M Muratori; N Tarozzi; F Carpentiero; S Danti; F M Perrone; M Cambi; A Casini; C Azzari; L Boni; M Maggi; A Borini; E Baldi Journal: Sci Rep Date: 2019-05-16 Impact factor: 4.379
Authors: Maria Eberhardt; Sylwia Prochowska; Anna M Duszewska; Ann Van Soom; Wanda Olech; Wojciech Niżański Journal: BMC Vet Res Date: 2022-08-10 Impact factor: 2.792
Authors: Bolan Yu; Hua Zhou; Min Liu; Ting Zheng; Lu Jiang; Mei Zhao; Xiaoxie Xu; Zhaofeng Huang Journal: PLoS One Date: 2015-12-28 Impact factor: 3.240
Authors: Monica Muratori; Nicoletta Tarozzi; Marta Cambi; Luca Boni; Anna Lisa Iorio; Claudia Passaro; Benedetta Luppino; Marco Nadalini; Sara Marchiani; Lara Tamburrino; Gianni Forti; Mario Maggi; Elisabetta Baldi; Andrea Borini Journal: Medicine (Baltimore) Date: 2016-05 Impact factor: 1.889