| Literature DB >> 15514040 |
E J Conley1, V Nduati, J L Gonzalez-Hernandez, A Mesfin, M Trudeau-Spanjers, S Chao, G R Lazo, D D Hummel, O D Anderson, L L Qi, B S Gill, B Echalier, A M Linkiewicz, J Dubcovsky, E D Akhunov, J Dvorák, J H Peng, N L V Lapitan, M S Pathan, H T Nguyen, X-F Ma, J P Gustafson, R A Greene, M E Sorrells, K G Hossain, V Kalavacharla, S F Kianian, D Sidhu, M Dilbirligi, K S Gill, D W Choi, R D Fenton, T J Close, P E McGuire, C O Qualset, J A Anderson.
Abstract
The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7.Entities:
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Year: 2004 PMID： 15514040 PMCID： PMC1448822 DOI： 10.1534/genetics.104.034801
Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562