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Literature DB >> 1549580

RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Abstract

The L-A double-stranded RNA virus of Saccharomyces cerevisiae makes a gag-pol fusion protein by a -1 ribosomal frameshift. The pol amino acid sequence includes consensus patterns typical of the RNA-dependent RNA polymerases (EC 2.7.7.48) of (+) strand and double-stranded RNA viruses of animals and plants. We have carried out "alanine-scanning mutagenesis" of the region of L-A including the two most conserved polymerase motifs, SG...T...NT..N (. = any amino acid) and GDD. By constructing and analyzing 46 different mutations in and around the RNA polymerase consensus regions, we have precisely defined the extent of domains and specific residues essential for viral replication. Assuming that this highly conserved region has a common secondary structure among different viruses, we predict a largely beta-sheet structure.

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Year: 1992 PMID： 1549580 PMCID： PMC48621 DOI： 10.1073/pnas.89.6.2185

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

36 in total

1. Portable encapsidation signal of the L-A double-stranded RNA virus of S. cerevisiae.

Authors: T Fujimura; R Esteban; L M Esteban; R B Wickner
Journal: Cell Date: 1990-08-24 Impact factor: 41.582

2. Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif.

Authors: S A Jablonski; M Luo; C D Morrow
Journal: J Virol Date: 1991-09 Impact factor: 5.103

3. An attempt to unify the structure of polymerases.

Authors: M Delarue; O Poch; N Tordo; D Moras; P Argos
Journal: Protein Eng Date: 1990-05

4. Gene overlap results in a viral protein having an RNA binding domain and a major coat protein domain.

Authors: T Fujimura; R B Wickner
Journal: Cell Date: 1988-11-18 Impact factor: 41.582

5. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

6. A sequence motif in many polymerases.

Authors: P Argos
Journal: Nucleic Acids Res Date: 1988-11-11 Impact factor: 16.971

7. Two chromosomal genes required for killing expression in killer strains of Saccharomyces cerevisiae.

Authors: R B Wickner; M J Leibowitz
Journal: Genetics Date: 1976-03-25 Impact factor: 4.562

8. Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae.

Authors: J E Hopper; K A Bostian; L B Rowe; D J Tipper
Journal: J Biol Chem Date: 1977-12-25 Impact factor: 5.157

9. Expression of yeast L-A double-stranded RNA virus proteins produces derepressed replication: a ski- phenocopy.

Authors: R B Wickner; T Icho; T Fujimura; W R Widner
Journal: J Virol Date: 1991-01 Impact factor: 5.103

10. A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein.

Authors: J D Dinman; T Icho; R B Wickner
Journal: Proc Natl Acad Sci U S A Date: 1991-01-01 Impact factor: 11.205

26 in total

Review 1. Yeast killer systems.

Authors: W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal: Clin Microbiol Rev Date: 1997-07 Impact factor: 26.132

2. Identification of cis-acting elements in the 3'-untranslated region of the dengue virus type 2 RNA that modulate translation and replication.

Authors: Mark Manzano; Erin D Reichert; Stephanie Polo; Barry Falgout; Wojciech Kasprzak; Bruce A Shapiro; Radhakrishnan Padmanabhan
Journal: J Biol Chem Date: 2011-04-22 Impact factor: 5.157

RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

1. Portable encapsidation signal of the L-A double-stranded RNA virus of S. cerevisiae.

2. Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif.

3. An attempt to unify the structure of polymerases.

4. Gene overlap results in a viral protein having an RNA binding domain and a major coat protein domain.

5. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

6. A sequence motif in many polymerases.

7. Two chromosomal genes required for killing expression in killer strains of Saccharomyces cerevisiae.

8. Translation of the L-species dsRNA genome of the killer-associated virus-like particles of Saccharomyces cerevisiae.

9. Expression of yeast L-A double-stranded RNA virus proteins produces derepressed replication: a ski- phenocopy.

10. A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein.

Review 1. Yeast killer systems.

2. Identification of cis-acting elements in the 3'-untranslated region of the dengue virus type 2 RNA that modulate translation and replication.

Review 3. Double-stranded RNA viruses of Saccharomyces cerevisiae.

4. Functions of conserved motifs in the RNA-dependent RNA polymerase of a yeast double-stranded RNA virus.

5. Evaluation of the roles of hydrophobic residues in the N-terminal region of archaeal trehalase in its folding.

6. A closely related group of RNA-dependent RNA polymerases from double-stranded RNA viruses.

7. Poly(A) polymerase contains multiple functional domains.

8. Saccharomyces cerevisiae L-BC double-stranded RNA virus replicase recognizes the L-A positive-strand RNA 3' end.

9. Mutational analysis of the conserved motifs of influenza A virus polymerase basic protein 1.

10. Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA.